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MB Sample ID: SA026803
Local Sample ID: | 1993041 |
Subject ID: | SU000537 |
Subject Type: | Animal |
Subject Species: | Macaca mulatta |
Taxonomy ID: | 9544 |
Age Or Age Range: | N/A| N/A| ~3years| ~3years| ~3years| ~3years| ~3years |
Weight Or Weight Range: | N/A| N/A| 6.05 kg| 6.05 kg| 6.05 kg| 6.05 kg| 6.05 kg |
Gender: | Male| Male| Male| Male| Male| Male| Male |
Subject Comments: | Transfusion Donor| Reference Animal| Experimental Subject|Experimental Subject| Experimental Subject| Experimental Subject| Experimental Subject |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000537 |
Subject Type: | Animal |
Subject Species: | Macaca mulatta |
Taxonomy ID: | 9544 |
Age Or Age Range: | N/A| N/A| ~3years| ~3years| ~3years| ~3years| ~3years |
Weight Or Weight Range: | N/A| N/A| 6.05 kg| 6.05 kg| 6.05 kg| 6.05 kg| 6.05 kg |
Gender: | Male| Male| Male| Male| Male| Male| Male |
Subject Comments: | Transfusion Donor| Reference Animal| Experimental Subject|Experimental Subject| Experimental Subject| Experimental Subject| Experimental Subject |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
1993041 | SA026803 | FL006777 | Male | Gender |
1993041 | SA026803 | FL006777 | None | Prior Infection |
1993041 | SA026803 | FL006777 | 19 | Days since Inoculation |
Collection:
Collection ID: | CO000531 |
Collection Summary: | Every Other Day Collection|Time Point 1 - Baseline: Uninfected control for each NHP.|Time Point 2 - Peak of infection: Peak of infection determined by clinical and parasitological assessment for each NHP. The time point is intended to characterize the host immune response and capture multi-omics data during the peak of parasitemias when the NHP is experiencing clinical signs of disease that may be severe or non-severe. NHPs were monitored, and clinical interventions were performed as necessary. Otherwise, NHPs self-resolved infection.|Time Point 3 - Post-Peak of Infection: Observations 7 days after peak infection.|Time Point 4 - 1st Relapse Peak of Infection: This time point tested expectations of a mild drop in hemoglobin levels and low parasitemias relative to peak infection arising from activation of hypnozoites. |Time Point 5 - Post-Peak of 1st Relapse Infection: Inter-relapse interval. |Time Point 6 - 2nd Relapse Peak of Infection: Same goal as time point 4 for 2nd relapse.|Time Point 7 - Post-Peak of 2nd Relapse Infection: Observations on NHP response after a second relapse infection. |
Sample Type: | Whole blood |
Collection Method: | Ear Prick|Intravenous|Intravenous|Intravenous|Intravenous|Intravenous|Intravenous|Intravenous |
Collection Time: | Every Other Day|Once|Once|Once|Once|Once|Once|Once |
Blood Serum Or Plasma: | Plasma |
Treatment:
Treatment ID: | TR000551 |
Treatment Summary: | Plasmodium Cynomolgi Strain B Sporozoites Inoculation |
Treatment: | Biotic |
Treatment Route: | Injection |
Sample Preparation:
Sampleprep ID: | SP000544 |
Sampleprep Summary: | aliquots of 65ul sample were mixed with 130ul acetonitrile and 3.25ul internal standard. Keep on ice and vortex every 15 minutes. Centrifuge for 10 minutes at 13.2rpm and 4C. Using a pipette, 130ul of supernatant was removed and placed into autosampler vials for the mass spectrometer. |
Sampleprep Protocol Filename: | Metab_Sample_Preparation_Plasma_serum_v1_May_17_2013.docx E4_E3_E18_E23_E24_E25_MalariaCore_Metabolomics_SOP_v1_6October2016.docx |
Processing Method: | Precipitation of protein, centrifuge, and remove supernatant |
Processing Storage Conditions: | on ice |
Extraction Method: | 1:2 sample:acetonitrile |
Extract Storage: | Pipette supernatant into autosampler vials for mass spectrometer |
Sample Spiking: | C18 standards: Caffeine and Diethyl-toluamide. Stable isotopes: [13C6]-D-glucose, [15N]-indole, [1,2-13C2]-palmitic acid, [15N,13C5]-L-methionine, [2-15N]-L-lysine dihydrochloride, [15N]-choline chloride, [13C5]-L-glutamic acid, [13C7]-benzoic acid, [15N]-L-tyrosine, [15N2]-uracil, [3,4-13C2]-cholesterol, [3,3-13C2]-cystine, [trimethyl-13C3]-caffeine, [U-13C5, U-15N2]-L-glutamine. |
Combined analysis:
Analysis ID | AN000788 | AN000789 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo QExactive | Thermo QExactive |
Column | Higgins C18 (100 x 2.1mm,5um) | Higgins C18 (100 x 2.1mm,5um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | peak intensity | peak intensity |
Chromatography:
Chromatography ID: | CH000565 |
Chromatography Summary: | untargeted chromatography (C18 column) |
Methods Filename: | Metab_Orbitrap_Setup_v1_May_17_2013.docx |
Chromatography Comments: | Thermo QExactive coupled to CTC PAL HTC Accela1-TMO autosampler |
Instrument Name: | Thermo QExactive |
Column Name: | Higgins C18 (100 x 2.1mm,5um) |
Column Pressure: | 200 Bar (max) |
Column Temperature: | 30 |
Flow Gradient: | A%: 0-10mins = 5%; B%: 0-2mins = 35%, 2-7mins = gradient to 95%, 7-10mins = 95%; C%: 0-2mins = 60%, 2-7mins = gradient to 0%, 7-10mins = 0% |
Flow Rate: | 0-2mins = 350 μL/min, 2-7mins = gradient to 400 μL/min, 7-10mins = 400 μL/min |
Injection Temperature: | 4C |
Internal Standard: | Caffeine, Diethyl-toluamide |
Internal Standard Mt: | 1.5 min; 6-6.6 min |
Sample Injection: | 10μL |
Sampling Cone: | ESI: HESI probe with S-lens combination |
Solvent A: | 100% water; 0.2% formic acid |
Solvent B: | 100% acetonitrile |
Analytical Time: | 10 minutes |
Capillary Voltage: | 4.6 kV |
Oven Temperature: | 45C |
Running Voltage: | 4.6 kV |
Weak Wash Solvent Name: | H20 with 10% methanol |
Weak Wash Volume: | 20μL |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000695 |
Analysis ID: | AN000788 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Capillary Temperature: | 250 |
Capillary Voltage: | 4.45 |
Collision Gas: | N2 |
Gas Pressure: | 50 PSI |
Ionization: | electrospray ionization |
Mass Accuracy: | 5 PPM |
Source Temperature: | 48 |
Spray Voltage: | 3.5 |
Atom Gun Current: | 1000000 |
Dataformat: | .raw,.cdf |
Probe Tip: | HESI |
Resolution Setting: | 120,000 |
Scan Range Moverz: | 85-1275 |
Analysis Protocol File: | Metabolomics_Core_Data_Analysis.docx |
MS ID: | MS000696 |
Analysis ID: | AN000789 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Capillary Temperature: | 250 |
Capillary Voltage: | 4.45 |
Collision Gas: | N2 |
Gas Pressure: | 50 PSI |
Ionization: | electrospray ionization |
Mass Accuracy: | 5 PPM |
Source Temperature: | 48 |
Spray Voltage: | 3.5 |
Atom Gun Current: | 1000000 |
Dataformat: | .raw,.cdf |
Probe Tip: | HESI |
Resolution Setting: | 120,000 |
Scan Range Moverz: | 85-1275 |
Analysis Protocol File: | Metabolomics_Core_Data_Analysis.docx |