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MB Sample ID: SA027179

Local Sample ID:ms5689-9
Subject ID:SU000541
Subject Type:Mouse
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

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Subject:

Subject ID:SU000541
Subject Type:Mouse
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
ms5689-9SA027179FL006850IRKOGroup
ms5689-9SA027179FL006850IR -/-Genotype
ms5689-9SA027179FL006850ChowDiet

Collection:

Collection ID:CO000535
Collection Summary:mouse muscle tissue
Sample Type:Muscle

Treatment:

Treatment ID:TR000555
Treatment Summary:Mice lacking insulin receptors (IR -/- genotype), or IGF-1 receptors (ICF-1 -/- genotype), or both were generated using Cre lox recombination. Controls were IR lox/lox, IGF-1 lox/lox, or both. Additional, 10 mice were included that were fed different diets for 8 weeks, chow or high fat diet.

Sample Preparation:

Sampleprep ID:SP000548
Sampleprep Summary:ceramide concentrations in muscle tissue

Combined analysis:

Analysis ID AN000793
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity
Column Waters Acquity BEH C8 (150 x 2mm,1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Thermo Quantiva QQQ
Ion Mode POSITIVE
Units ng/mg

Chromatography:

Chromatography ID:CH000569
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH C8 (150 x 2mm,1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000700
Analysis ID:AN000793
Instrument Name:Thermo Quantiva QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
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