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MB Sample ID: SA027997
Local Sample ID: | 325103 |
Subject ID: | SU000563 |
Subject Type: | Animal |
Subject Species: | Canis lupus familiaris |
Taxonomy ID: | 9615 |
Age Or Age Range: | 1 year - 15 years |
Weight Or Weight Range: | 5 kg - 40 kg |
Gender: | Male Neutered or Female Spayed |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000563 |
Subject Type: | Animal |
Subject Species: | Canis lupus familiaris |
Taxonomy ID: | 9615 |
Age Or Age Range: | 1 year - 15 years |
Weight Or Weight Range: | 5 kg - 40 kg |
Gender: | Male Neutered or Female Spayed |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
325103 | SA027997 | FL006939 | Diabetic | Health Status |
325103 | SA027997 | FL006939 | Neutered male | Gender |
325103 | SA027997 | FL006939 | Fasted | Fasting Status |
325103 | SA027997 | FL006939 | Mixed | Breed |
325103 | SA027997 | FL006939 | Large | Size (S/M/L) |
Collection:
Collection ID: | CO000557 |
Collection Summary: | Blood collected by peripheral venipuncture into red top tube (containing clot activator). Blood was allowed to clot, then centrifuged at 2000 g x 7 minutes between 20-30 minutes after sample collection. Serum was frozen immediately after separation (always within 1 hour of collection) at -80 C. |
Sample Type: | Blood (serum) |
Collection Method: | Peripheral venipuncture |
Collection Location: | Jugular, saphenous, or cephalic vein |
Collection Frequency: | Once |
Storage Conditions: | Room temperature until centrifugation. Then -80 C immediately after separation from red cells. Serum was thawed once on ice, 100 uL aliquots taken, and aliquots re-frozen (at -80C) until use for metabolomics. |
Collection Vials: | Red top with clot activator |
Storage Vials: | Cryovials (polypropylene with silicone gasket - Fisher Brand) |
Collection Tube Temp: | Room temperature |
Blood Serum Or Plasma: | Serum |
Treatment:
Treatment ID: | TR000577 |
Treatment Summary: | None |
Sample Preparation:
Sampleprep ID: | SP000570 |
Sampleprep Summary: | See protocol |
Sampleprep Protocol Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
Combined analysis:
Analysis ID | AN000822 | AN000823 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Scientific Q Exactive | Thermo Scientific Q Exactive |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak Area | Peak Area |
Chromatography:
Chromatography ID: | CH000587 |
Chromatography Summary: | see protocol |
Instrument Name: | Thermo Scientific Q Exactive |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000723 |
Analysis ID: | AN000822 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |
MS ID: | MS000724 |
Analysis ID: | AN000823 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |