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MB Sample ID: SA028745
Local Sample ID: | kim_AiPSC_19 |
Subject ID: | SU000576 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Age Or Age Range: | B6/CBA |
Cell Primary Immortalized: | B6/CBA |
Cell Passage Number: | Cultured primary cells |
Cell Counts: | 16-18 |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000576 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Age Or Age Range: | B6/CBA |
Cell Primary Immortalized: | B6/CBA |
Cell Passage Number: | Cultured primary cells |
Cell Counts: | 16-18 |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
kim_AiPSC_19 | SA028745 | FL007014 | Untreated | Treatment |
Collection:
Collection ID: | CO000570 |
Collection Summary: | none |
Collection Protocol Comments: | Cells were scraped off on ice in pbs |
Sample Type: | Cultured primary cells |
Collection Location: | Cold Room |
Collection Frequency: | two plates at a time |
Collection Duration: | 15 mins |
Volumeoramount Collected: | In 5ml cold PBS |
Storage Conditions: | N2 snap frozen, stored at -80 |
Collection Vials: | Falcon tube and eppendorf tube |
Storage Vials: | Eppendorf tube |
Collection Tube Temp: | <4C |
Additives: | None |
Tissue Cell Quantity Taken: | 5-10mill |
Treatment:
Treatment ID: | TR000590 |
Treatment Summary: | No treatment |
Plant Humidity: | - |
Sample Preparation:
Sampleprep ID: | SP000583 |
Sampleprep Summary: | None |
Sampleprep Protocol Filename: | Metabolomics_Global_LCMS.pdf |
Combined analysis:
Analysis ID | AN000849 | AN000850 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | None | None |
Column | None | None |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak height | Peak height |
Chromatography:
Chromatography ID: | CH000606 |
Methods Filename: | Metabolomics_Global_LCMS.pdf |
Instrument Name: | None |
Column Name: | None |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000750 |
Analysis ID: | AN000849 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | HESI |
Ion Mode: | POSITIVE |
MS ID: | MS000751 |
Analysis ID: | AN000850 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | HESI |
Ion Mode: | NEGATIVE |