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MB Sample ID: SA028765
Local Sample ID: | M15A |
Subject ID: | SU000578 |
Subject Type: | Larval fish at one month |
Subject Species: | Danio rerio |
Taxonomy ID: | 7955 |
Species Group: | Fish |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000578 |
Subject Type: | Larval fish at one month |
Subject Species: | Danio rerio |
Taxonomy ID: | 7955 |
Species Group: | Fish |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
M15A | SA028765 | FL007019 | DEHP | Chemical Treatment |
Collection:
Collection ID: | CO000572 |
Collection Summary: | Expt 2: Larval zebrafish were exposed to a plastic for 96 hours. Fish were pooled into groups of 5 (10 biological replicates derived from 10 independent technical replicates. |
Sample Type: | Whole larval fish. |
Treatment:
Treatment ID: | TR000592 |
Treatment Summary: | Expt 2: Larval zebrafish were exposed to a plastic for 96 hours. |
Treatment Compound: | DEHP (a plastic) |
Treatment Route: | waterborne |
Treatment Dose: | 10 uM DEHP |
Treatment Doseduration: | 96 hours |
Treatment Vehicle: | DMSO |
Animal Vet Treatments: | None |
Animal Anesthesia: | Tricaine |
Animal Fasting: | Early stage larval fish, do not feed for first 144 hours. |
Animal Endp Euthanasia: | MS-222 or Tricaine |
Animal Endp Tissue Coll List: | Whole larval fish placed into a vial. Five fish per vial. |
Animal Endp Clinical Signs: | No clinical signs |
Sample Preparation:
Sampleprep ID: | SP000585 |
Sampleprep Summary: | None |
Sampleprep Protocol Filename: | Metabolomics_Global_LCMS.pdf Metabolomics_LCMSProtocol_plasma.pdf |
Combined analysis:
Analysis ID | AN000853 | AN000854 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Scientific-Dionex Ultimate 3000 | Thermo Scientific-Dionex Ultimate 3000 |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak height | Peak height |
Chromatography:
Chromatography ID: | CH000608 |
Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000754 |
Analysis ID: | AN000853 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Analysis Protocol File: | Metabolomics_Global_LCMS.pdf |
MS ID: | MS000755 |
Analysis ID: | AN000854 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | Metabolomics_Global_LCMS.pdf |