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MB Sample ID: SA029066
| Local Sample ID: | M87 |
| Subject ID: | SU000587 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Both |
| Human Trial Type: | validation/discovery trial |
| Human Inclusion Criteria: | All subjects will receive hemodialysis in-center on a 3 times per week dialysis schedule. Subjects are eligible to participate if 1) subjects that have received EPO will have received it for 6 or more months, 2) between the ages of 18 and 80 years, 3) have an adequacy of dialysis (Kt/V) that is ≥ 1.2, 4) have adequate iron stores as measured by ferritin and transferrin saturation. |
| Human Exclusion Criteria: | None |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000587 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Both |
| Human Trial Type: | validation/discovery trial |
| Human Inclusion Criteria: | All subjects will receive hemodialysis in-center on a 3 times per week dialysis schedule. Subjects are eligible to participate if 1) subjects that have received EPO will have received it for 6 or more months, 2) between the ages of 18 and 80 years, 3) have an adequacy of dialysis (Kt/V) that is ≥ 1.2, 4) have adequate iron stores as measured by ferritin and transferrin saturation. |
| Human Exclusion Criteria: | None |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| M87 | SA029066 | FL007057 | F | Gender |
| M87 | SA029066 | FL007057 | - | Race |
Collection:
| Collection ID: | CO000581 |
| Collection Summary: | Subjects that passed the screening were asked to donate 20 mL of blood for analysis following an informed consent process. The blood was processed to plasma and serum and stored as 0.5mL aliquots at -70 degrees until analyzed. |
| Sample Type: | Blood |
| Additives: | EDTA |
| Blood Serum Or Plasma: | Plasma |
Treatment:
| Treatment ID: | TR000601 |
| Treatment Summary: | NA |
Sample Preparation:
| Sampleprep ID: | SP000594 |
| Sampleprep Summary: | A volume of 150 µL of each EDTA-plasma sample was dispensed into an acid-washed plastic digestion tube. To each digestion vessel, 300 µL of concentrated HNO3, 150 µL of concentrated HCl, and 100 µL of H2O2 solution was added. Samples were loosely capped and placed in the graphite digestion block and heated at a temperature of 90 °C for 2 hours. At the end of digestion, all samples were removed from the heating block and allowed to cool to room temperature. In the clean hood, samples were spiked with a multielement internal standard to provide a final concentration of 1.0 ng/mL Sc, In, and Pr, and diluted to the final volume with deionized (DI) H2O. |
| Processing Method: | Acid Digestion |
| Processing Storage Conditions: | 90°C |
| Extract Storage: | 8°C |
| Sample Spiking: | Sc, In, Pr |
Combined analysis:
| Analysis ID | AN000868 |
|---|---|
| Analysis type | MS |
| Chromatography type | ICP |
| Chromatography system | Thermo Element 2 ICP-MS |
| Column | None |
| MS Type | Other |
| MS instrument type | Sector Field |
| MS instrument name | Thermo Element 2 ICP |
| Ion Mode | POSITIVE |
| Units | ng/mL plasma |
Chromatography:
| Chromatography ID: | CH000617 |
| Instrument Name: | Thermo Element 2 ICP-MS |
| Column Name: | None |
| Chromatography Type: | ICP |
MS:
| MS ID: | MS000769 |
| Analysis ID: | AN000868 |
| Instrument Name: | Thermo Element 2 ICP |
| Instrument Type: | Sector Field |
| MS Type: | Other |
| Ion Mode: | POSITIVE |
| Mass Accuracy: | 7000 (medium resolution) and 10000 (high resolution) |
| Dataformat: | .dat |
| Scan Range Moverz: | 27-209 |
