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MB Sample ID: SA029948
Local Sample ID: | PJH_006 |
Subject ID: | SU000593 |
Subject Type: | Animal |
Subject Species: | Bos taurus |
Taxonomy ID: | 9913 |
Genotype Strain: | Holstein |
Age Or Age Range: | Adult |
Gender: | Female |
Animal Animal Supplier: | University of Florida |
Animal Housing: | Pasture |
Animal Light Cycle: | Natural |
Animal Feed: | Pasture |
Animal Water: | Ad Lib |
Animal Inclusion Criteria: | Cyclic |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000593 |
Subject Type: | Animal |
Subject Species: | Bos taurus |
Taxonomy ID: | 9913 |
Genotype Strain: | Holstein |
Age Or Age Range: | Adult |
Gender: | Female |
Animal Animal Supplier: | University of Florida |
Animal Housing: | Pasture |
Animal Light Cycle: | Natural |
Animal Feed: | Pasture |
Animal Water: | Ad Lib |
Animal Inclusion Criteria: | Cyclic |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
PJH_006 | SA029948 | FL007101 | D3 | Days after ovulation |
Collection:
Collection ID: | CO000587 |
Collection Summary: | Recover uterus at slaughter; flush uterine lumen with 30 ml DPBS |
Sample Type: | Uterine fluid |
Collection Method: | Flush |
Volumeoramount Collected: | 30 ml |
Storage Conditions: | -20 C |
Treatment:
Treatment ID: | TR000607 |
Treatment Summary: | Uterine flushings collected at Day 0, 3, 5 and 7 after estrus |
Sample Preparation:
Sampleprep ID: | SP000600 |
Sampleprep Summary: | None |
Sampleprep Protocol Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
Combined analysis:
Analysis ID | AN000879 | AN000880 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Scientific-Dionex Ultimate 3000 | Thermo Scientific-Dionex Ultimate 3000 |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak height | Peak height |
Chromatography:
Chromatography ID: | CH000624 |
Methods Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
Chromatography Comments: | Ensure that Column is an ACE Excel 2 C18-PFP with dimensions of 100 x 2.1mm, 2.0 μm with a Halo C18-PFP guard attached |
Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Flow Rate: | 0.350mL/min-0.600mL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000780 |
Analysis ID: | AN000879 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |
MS ID: | MS000781 |
Analysis ID: | AN000880 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |