Return to study ST000572 main page
MB Sample ID: SA029972
Local Sample ID: | SPM2 |
Subject ID: | SU000594 |
Subject Type: | bacterial microbial community |
Subject Species: | Mixed |
Species Group: | Microorganism |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000594 |
Subject Type: | bacterial microbial community |
Subject Species: | Mixed |
Species Group: | Microorganism |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
SPM2 | SA029972 | FL007105 | pustular stromatolite forming mat | microbial community type |
Collection:
Collection ID: | CO000588 |
Collection Summary: | field collected samples from Shark Bay, Australia |
Collection Protocol Filename: | Shark Bay microbial mats |
Collection Protocol Comments: | collected using sterile scalpel and placed immediatelyl into RNAlater - frozen at -20C in field (2 weeks) - back in lab stored at -80C |
Sample Type: | microbial community - biofilm |
Collection Location: | Hamelin Pool, Shark Bay Western Australia |
Collection Time: | 12PM |
Storage Conditions: | RNALater |
Collection Vials: | nalgene container |
Treatment:
Treatment ID: | TR000608 |
Treatment Summary: | no treatment; natural field collected samples |
Sample Preparation:
Sampleprep ID: | SP000601 |
Sampleprep Summary: | None |
Sampleprep Protocol Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
Combined analysis:
Analysis ID | AN000881 | AN000882 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Scientific-Dionex Ultimate 3000 | Thermo Scientific-Dionex Ultimate 3000 |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | peak height | peak height |
Chromatography:
Chromatography ID: | CH000625 |
Methods Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Flow Rate: | 0.350mL/min-0.600mL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000782 |
Analysis ID: | AN000881 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |
MS ID: | MS000783 |
Analysis ID: | AN000882 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |