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MB Sample ID: SA030606
Local Sample ID: | 2015799 |
Subject ID: | SU000601 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Subject Comments: | 220 subjects |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000601 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Subject Comments: | 220 subjects |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
2015799 | SA030606 | FL007127 | P.Vivax | Current Malaria Infection |
2015799 | SA030606 | FL007127 | NO | Prior Malaria Infection |
2015799 | SA030606 | FL007127 | N/A | Chloroquine Resistance |
Collection:
Collection ID: | CO000595 |
Collection Summary: | none |
Sample Type: | Blood |
Collection Frequency: | Once or Twice |
Treatment:
Treatment ID: | TR000615 |
Treatment Summary: | None |
Sample Preparation:
Sampleprep ID: | SP000608 |
Sampleprep Summary: | aliquots of 30ul sample were mixed with 100ul acetonitrile and 2.5ul internal standard. Keep on ice for 30 minutes and vortex every 15 minutes. Centrifuge for 10 minutes at 13.2rpm and 4C. Using a pipette, 100ul of supernatant was removed and placed into autosampler vials for the mass spectrometer. |
Sampleprep Protocol Filename: | Metab_Sample_Preparation_Plasma_serum_v1_May_17_2013.docx |
Processing Method: | Precipitation of protein, centrifuge, and remove supernatant |
Processing Storage Conditions: | on ice |
Extraction Method: | 1:2 sample:acetonitrile |
Extract Storage: | Pipette supernatant into autosampler vials for mass spectrometer |
Sample Spiking: | C18 standards: Caffeine and Diethyl-toluamide. Stable isotopes: [13C6]-D-glucose, [15N]-indole, [1,2-13C2]-palmitic acid, [15N,13C5]-L-methionine, [2-15N]-L-lysine dihydrochloride, [15N]-choline chloride, [13C5]-L-glutamic acid, [13C7]-benzoic acid, [15N]-L-tyrosine, [15N2]-uracil, [3,4-13C2]-cholesterol, [3,3-13C2]-cystine, [trimethyl-13C3]-caffeine, [U-13C5, U-15N2]-L-glutamine. |
Combined analysis:
Analysis ID | AN000888 | AN000889 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Ion exchange |
Chromatography system | Thermo Accela CTC | Thermo Accela CTC |
Column | Thermo Higgins C18 (100 x 2.1mm,2.6um) | Hamilton Anion Exchange HPLCs,100x2.1,2.6m,Thermo Fisher |
MS Type | ESI | ESI |
MS instrument type | Single quadrupole | Single quadrupole |
MS instrument name | Thermo LTQ-FT | Thermo LTQ-FT |
Ion Mode | POSITIVE | POSITIVE |
Units | unspecified | unspecified |
Chromatography:
Chromatography ID: | CH000631 |
Chromatography Summary: | Positive untargeted chromatography (C18 column) |
Methods Filename: | Metab_Orbitrap_Setup_v2_26Jun2013.docx |
Instrument Name: | Thermo Accela CTC |
Column Name: | Thermo Higgins C18 (100 x 2.1mm,2.6um) |
Column Temperature: | 30 |
Flow Gradient: | A: 0-2mins = 90%, 2-7mins = gradient to 0%, 7-10mins = 0%. B: 0-2mins = 5%, 2-7mins = gradient to 95%, 7-10mins = 95%. C: 0-10mins = 5% |
Flow Rate: | 350uL/min |
Internal Standard: | Caffeine, Diethyl-toluamide |
Internal Standard Mt: | 1.5 min, 6-6.6 min |
Sample Injection: | 10uL |
Sampling Cone: | HESI probe with S-lens combination for ESI |
Solvent A: | 100% water |
Solvent B: | 100% acetonitrile |
Analytical Time: | 10 minutes |
Capillary Voltage: | 4.6 kV |
Oven Temperature: | 45 C |
Running Voltage: | 45 |
Weak Wash Solvent Name: | H20 with 10% methanol |
Sample Syringe Size: | 20uL |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH000632 |
Chromatography Summary: | Positive untargeted anion exchange chromatography (AE column) |
Methods Filename: | Metab_Orbitrap_Setup_v1_May_17_2013.docx |
Instrument Name: | Thermo Accela CTC |
Column Name: | Hamilton Anion Exchange HPLCs,100x2.1,2.6m,Thermo Fisher |
Column Temperature: | 30 |
Flow Gradient: | A: 0-2mins = 10%, 2-7mins = gradient to 80%, 7-10mins = 80%. B: 0-2mins = 80%, 2-7mins = gradient to 10%, 7-10mins = 10%. C: 0-10mins = 10% |
Flow Rate: | 350uL/min |
Internal Standard: | [13C6]-D-glucose, [15N]-indole, [1,2-13C2]-palmitic acid, [15N,13C5]-L-methionine, [2-15N]-L-lysine dihydrochloride, [15N]-choline chloride, [13C5]-L-glutamic acid, [13C7]-benzoic acid, [15N]-L-tyrosine, [15N2]-uracil, [3,4-13C2]-cholesterol, [3,3-13C2]-cystine, [trimethyl-13C3]-caffeine, [U-13C5, U-15N2]-L-glutamine |
Sample Injection: | 10uL |
Sampling Cone: | HESI probe with S-lens combination for ESI |
Solvent A: | 100% water |
Solvent B: | 100% acetonitrile |
Analytical Time: | 10 minutes |
Capillary Voltage: | 4.6 kV |
Oven Temperature: | 45 C |
Running Voltage: | 45 |
Weak Wash Solvent Name: | H20 with 10% methanol |
Sample Syringe Size: | 20uL |
Chromatography Type: | Ion exchange |
MS:
MS ID: | MS000790 |
Analysis ID: | AN000888 |
Instrument Name: | Thermo LTQ-FT |
Instrument Type: | Single quadrupole |
MS Type: | ESI |
MS Comments: | C18 Pos: technical triplicates for each sample |
Ion Mode: | POSITIVE |
Capillary Voltage: | 4.45 |
Collision Gas: | N2 |
Gas Pressure: | 50 |
Ionization: | electrospray ionization |
Mass Accuracy: | 5 ppm |
Source Temperature: | 48 |
Spray Voltage: | 3.5 |
Atom Gun Current: | 1000000 |
Dataformat: | .raw, .cdf |
Probe Tip: | HESI |
Resolution Setting: | 60000 |
Scan Range Moverz: | 85-2000 |
Analysis Protocol File: | Metab_Orbitrap_Setup_v2_26Jun2013.docx |
MS ID: | MS000791 |
Analysis ID: | AN000889 |
Instrument Name: | Thermo LTQ-FT |
Instrument Type: | Single quadrupole |
MS Type: | ESI |
MS Comments: | Anion exchange Pos: technical triplicates for each sample |
Ion Mode: | POSITIVE |
Capillary Voltage: | 4.45 |
Collision Gas: | N2 |
Gas Pressure: | 50 |
Ionization: | electrospray ionization |
Mass Accuracy: | 5 ppm |
Source Temperature: | 48 |
Spray Voltage: | 3.5 |
Atom Gun Current: | 1000000 |
Dataformat: | .raw, .cdf |
Probe Tip: | HESI |
Resolution Setting: | 60000 |
Scan Range Moverz: | 85-2000 |
Analysis Protocol File: | Metab_Orbitrap_Setup_v2_26Jun2013.docx |