Return to study ST000578 main page

MB Sample ID: SA030630

Local Sample ID:2015984
Subject ID:SU000601
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Subject Comments:220 subjects
Species Group:Human

Select appropriate tab below to view additional metadata details:

Subject:

Subject ID:SU000601
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Subject Comments:220 subjects
Species Group:Human

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
2015984SA030630FL007129P.VivaxCurrent Malaria Infection
2015984SA030630FL007129YESPrior Malaria Infection
2015984SA030630FL007129N/AChloroquine Resistance

Collection:

Collection ID:CO000595
Collection Summary:none
Sample Type:Blood
Collection Frequency:Once or Twice

Treatment:

Treatment ID:TR000615
Treatment Summary:None

Sample Preparation:

Sampleprep ID:SP000608
Sampleprep Summary:aliquots of 30ul sample were mixed with 100ul acetonitrile and 2.5ul internal standard. Keep on ice for 30 minutes and vortex every 15 minutes. Centrifuge for 10 minutes at 13.2rpm and 4C. Using a pipette, 100ul of supernatant was removed and placed into autosampler vials for the mass spectrometer.
Sampleprep Protocol Filename:Metab_Sample_Preparation_Plasma_serum_v1_May_17_2013.docx
Processing Method:Precipitation of protein, centrifuge, and remove supernatant
Processing Storage Conditions:on ice
Extraction Method:1:2 sample:acetonitrile
Extract Storage:Pipette supernatant into autosampler vials for mass spectrometer
Sample Spiking:C18 standards: Caffeine and Diethyl-toluamide. Stable isotopes: [13C6]-D-glucose, [15N]-indole, [1,2-13C2]-palmitic acid, [15N,13C5]-L-methionine, [2-15N]-L-lysine dihydrochloride, [15N]-choline chloride, [13C5]-L-glutamic acid, [13C7]-benzoic acid, [15N]-L-tyrosine, [15N2]-uracil, [3,4-13C2]-cholesterol, [3,3-13C2]-cystine, [trimethyl-13C3]-caffeine, [U-13C5, U-15N2]-L-glutamine.

Combined analysis:

Analysis ID AN000888 AN000889
Analysis type MS MS
Chromatography type Reversed phase Ion exchange
Chromatography system Thermo Accela CTC Thermo Accela CTC
Column Thermo Higgins C18 (100 x 2.1mm,2.6um) Hamilton Anion Exchange HPLCs,100x2.1,2.6m,Thermo Fisher
MS Type ESI ESI
MS instrument type Single quadrupole Single quadrupole
MS instrument name Thermo LTQ-FT Thermo LTQ-FT
Ion Mode POSITIVE POSITIVE
Units unspecified unspecified

Chromatography:

Chromatography ID:CH000631
Chromatography Summary:Positive untargeted chromatography (C18 column)
Methods Filename:Metab_Orbitrap_Setup_v2_26Jun2013.docx
Instrument Name:Thermo Accela CTC
Column Name:Thermo Higgins C18 (100 x 2.1mm,2.6um)
Column Temperature:30
Flow Gradient:A: 0-2mins = 90%, 2-7mins = gradient to 0%, 7-10mins = 0%. B: 0-2mins = 5%, 2-7mins = gradient to 95%, 7-10mins = 95%. C: 0-10mins = 5%
Flow Rate:350uL/min
Internal Standard:Caffeine, Diethyl-toluamide
Internal Standard Mt:1.5 min, 6-6.6 min
Sample Injection:10uL
Sampling Cone:HESI probe with S-lens combination for ESI
Solvent A:100% water
Solvent B:100% acetonitrile
Analytical Time:10 minutes
Capillary Voltage:4.6 kV
Oven Temperature:45 C
Running Voltage:45
Weak Wash Solvent Name:H20 with 10% methanol
Sample Syringe Size:20uL
Chromatography Type:Reversed phase
  
Chromatography ID:CH000632
Chromatography Summary:Positive untargeted anion exchange chromatography (AE column)
Methods Filename:Metab_Orbitrap_Setup_v1_May_17_2013.docx
Instrument Name:Thermo Accela CTC
Column Name:Hamilton Anion Exchange HPLCs,100x2.1,2.6m,Thermo Fisher
Column Temperature:30
Flow Gradient:A: 0-2mins = 10%, 2-7mins = gradient to 80%, 7-10mins = 80%. B: 0-2mins = 80%, 2-7mins = gradient to 10%, 7-10mins = 10%. C: 0-10mins = 10%
Flow Rate:350uL/min
Internal Standard:[13C6]-D-glucose, [15N]-indole, [1,2-13C2]-palmitic acid, [15N,13C5]-L-methionine, [2-15N]-L-lysine dihydrochloride, [15N]-choline chloride, [13C5]-L-glutamic acid, [13C7]-benzoic acid, [15N]-L-tyrosine, [15N2]-uracil, [3,4-13C2]-cholesterol, [3,3-13C2]-cystine, [trimethyl-13C3]-caffeine, [U-13C5, U-15N2]-L-glutamine
Sample Injection:10uL
Sampling Cone:HESI probe with S-lens combination for ESI
Solvent A:100% water
Solvent B:100% acetonitrile
Analytical Time:10 minutes
Capillary Voltage:4.6 kV
Oven Temperature:45 C
Running Voltage:45
Weak Wash Solvent Name:H20 with 10% methanol
Sample Syringe Size:20uL
Chromatography Type:Ion exchange

MS:

MS ID:MS000790
Analysis ID:AN000888
Instrument Name:Thermo LTQ-FT
Instrument Type:Single quadrupole
MS Type:ESI
MS Comments:C18 Pos: technical triplicates for each sample
Ion Mode:POSITIVE
Capillary Voltage:4.45
Collision Gas:N2
Gas Pressure:50
Ionization:electrospray ionization
Mass Accuracy:5 ppm
Source Temperature:48
Spray Voltage:3.5
Atom Gun Current:1000000
Dataformat:.raw, .cdf
Probe Tip:HESI
Resolution Setting:60000
Scan Range Moverz:85-2000
Analysis Protocol File:Metab_Orbitrap_Setup_v2_26Jun2013.docx
  
MS ID:MS000791
Analysis ID:AN000889
Instrument Name:Thermo LTQ-FT
Instrument Type:Single quadrupole
MS Type:ESI
MS Comments:Anion exchange Pos: technical triplicates for each sample
Ion Mode:POSITIVE
Capillary Voltage:4.45
Collision Gas:N2
Gas Pressure:50
Ionization:electrospray ionization
Mass Accuracy:5 ppm
Source Temperature:48
Spray Voltage:3.5
Atom Gun Current:1000000
Dataformat:.raw, .cdf
Probe Tip:HESI
Resolution Setting:60000
Scan Range Moverz:85-2000
Analysis Protocol File:Metab_Orbitrap_Setup_v2_26Jun2013.docx
  logo