Return to study ST000580 main page
MB Sample ID: SA030737
Local Sample ID: | DBA2J 10M FAHL3 |
Subject ID: | SU000603 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | DBA/2J |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000603 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | DBA/2J |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
DBA2J 10M FAHL3 | SA030737 | FL007139 | AH | Tissue Location |
DBA2J 10M FAHL3 | SA030737 | FL007139 | Female | Sex |
DBA2J 10M FAHL3 | SA030737 | FL007139 | Hypertensive | Phenotype |
Collection:
Collection ID: | CO000597 |
Collection Summary: | DBA/2J mice were anesthetized by an intraperitoneal injection of a cocktail of Ketamine and Xylazine. A drop of tetracaine hydrochloride ophthalmic solution (0.5%, Bausch & Lomb, USA) was used to anesthetize the ocular surface prior to AH procurement. Normotensive and hypertensive AH was obtained from the anterior chamber of DBA/2J mice via paracentesis using a syringe (catalog no. 7635-01 Hamilton, Reno, Nevada) with a small 33 gauge removable needle (701RN, catalog no. 7803-05 Hamilton). TM samples were obtained through surgical removal after euthanization with carbon dioxide and adhering to animal procedures approved by the IACUC. |
Sample Type: | Eye tissue |
Treatment:
Treatment ID: | TR000617 |
Treatment Summary: | No treatment was performed on the mice. |
Sample Preparation:
Sampleprep ID: | SP000610 |
Sampleprep Summary: | A total of ~3 μl of AH and ∼0.5-1 mm3 of TM per mice was used for phospholipid extraction using modified Bligh and Dyer extraction method[13,14]. Phospholipids extracted in the lower organic phase were dried using a Speed-Vac (Model 7810014; Labconco, Kansas City, MO), and then flushed with argon gas for oxidation prevention. Proteins procured from the upper aqueous phase were quantified using a suitable modification of Bradford’s method. |
Combined analysis:
Analysis ID | AN000891 |
---|---|
Analysis type | MS |
Chromatography type | None (Direct infusion) |
Chromatography system | TSQ Quantum Access Max |
Column | None |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Thermo Quantiva Access Max |
Ion Mode | NEGATIVE |
Units | pmol/ ug of protein |
Chromatography:
Chromatography ID: | CH000634 |
Instrument Name: | TSQ Quantum Access Max |
Column Name: | None |
Chromatography Type: | None (Direct infusion) |
MS:
MS ID: | MS000793 |
Analysis ID: | AN000891 |
Instrument Name: | Thermo Quantiva Access Max |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | NEGATIVE |