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MB Sample ID: SA030760
| Local Sample ID: | DBA2J 7.5M FAH2L6 |
| Subject ID: | SU000604 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | DBA/2J |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000604 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | DBA/2J |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| DBA2J 7.5M FAH2L6 | SA030760 | FL007148 | AH | Tissue Location |
| DBA2J 7.5M FAH2L6 | SA030760 | FL007148 | Female | Sex |
| DBA2J 7.5M FAH2L6 | SA030760 | FL007148 | Normotensive | Phenotype |
Collection:
| Collection ID: | CO000598 |
| Collection Summary: | DBA/2J mice were anesthetized by an intraperitoneal injection of a cocktail of Ketamine and Xylazine. A drop of tetracaine hydrochloride ophthalmic solution (0.5%, Bausch & Lomb, USA) was used to anesthetize the ocular surface prior to AH procurement. Normotensive and hypertensive AH was obtained from the anterior chamber of DBA/2J mice via paracentesis using a syringe (catalog no. 7635-01 Hamilton, Reno, Nevada) with a small 33 gauge removable needle (701RN, catalog no. 7803-05 Hamilton). TM samples were obtained through surgical removal after euthanization with carbon dioxide and adhering to animal procedures approved by the IACUC. |
| Sample Type: | Eye tissue |
Treatment:
| Treatment ID: | TR000618 |
| Treatment Summary: | No treatment was performed on the mice. |
Sample Preparation:
| Sampleprep ID: | SP000611 |
| Sampleprep Summary: | A total of ~3 μl of AH and ∼0.5-1 mm3 of TM per mice was used for phospholipid extraction using modified Bligh and Dyer extraction method[13,14]. Phospholipids extracted in the lower organic phase were dried using a Speed-Vac (Model 7810014; Labconco, Kansas City, MO), and then flushed with argon gas for oxidation prevention. Proteins procured from the upper aqueous phase were quantified using a suitable modification of Bradford’s method. |
Combined analysis:
| Analysis ID | AN000892 |
|---|---|
| Analysis type | MS |
| Chromatography type | None (Direct infusion) |
| Chromatography system | TSQ Quantum Access Max |
| Column | None |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Thermo Quantiva Access Max |
| Ion Mode | NEGATIVE |
| Units | pmol/ ug of protein |
Chromatography:
| Chromatography ID: | CH000635 |
| Instrument Name: | TSQ Quantum Access Max |
| Column Name: | None |
| Chromatography Type: | None (Direct infusion) |
MS:
| MS ID: | MS000794 |
| Analysis ID: | AN000892 |
| Instrument Name: | Thermo Quantiva Access Max |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
