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MB Sample ID: SA031818
Local Sample ID: | C18 |
Subject ID: | SU000610 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000610 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
C18 | SA031818 | FL007196 | control | factor |
Collection:
Collection ID: | CO000604 |
Collection Summary: | The samples were collected in Dr. Bruce Johnson lab, at the Department of Cardiovascular Diseases, Mayo Clinic. EBC samples were collected using Jaeger EcoScreen cooling unit (-20°C). Patients were breathing through mouth piece while wearing a nose clip during 30 minutes. Saliva was collected from the same patients during last 15 minutes. The samples were frozen immediately after collection. |
Sample Type: | Breath |
Treatment:
Treatment ID: | TR000624 |
Treatment Summary: | We will focus initially on patients with classic systolic HF who are admitted to St Mary’s Hospital with decompensated disease. These patients are currently being studied to quantify changes in lung water during the “recompensation” diuresis in the hospital and the related changes in alveolar gas conductance and breathing pattern to develop a predictive algorithm for likelihood of readmission, but also to obtain a better understanding of fluid regulation in the lungs. As these subjects are recruited, we will recruit age and sex matched stable HF patients going through similar testing in parallel running studies as well as age and sex matched control subjects. We will target 12 subjects per group and in addition to the EBC collection we will obtain saliva samples and blood at the same time points. |
Sample Preparation:
Sampleprep ID: | SP000617 |
Sampleprep Summary: | EBC: Transfer 800μl aliquots of EBC samples into a 1.5ml microcentrifuge tubes and dry down (lyophilize) each sample in a centrifugal vacuum evaporator for 4-6 h. Apply no heating during the drying process. In the dried EBC samples add 250μl of phosphate buffer and 250μl of 4mM TSP-d4 solution. Vortex for 20 s. Transfer the solution to 5mm NMR tube. |
Analysis:
MB Sample ID: | SA031818 |
Analysis ID: | AN000902 |
Analysis Type: | NMR |
Num Factors: | 2 |
Num Metabolites: | 9 |
Units: | uM |
NMR:
NMR ID: | NM000100 |
Analysis ID: | AN000902 |
Instrument Name: | Bruker 600 MHz Avance III HD spectrometer |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D 1H |
Spectrometer Frequency: | 600 MHz |