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MB Sample ID: SA033435
Local Sample ID: | CCMB12WB |
Subject ID: | SU000628 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | Healthy - Mean (±SD): 54.9±8.2 years | Sepsis - Mean (+SD): 54.7±17.3 years |
Weight Or Weight Range: | Healthy - Mean (+SD): 74.4±1.71 kg; BMI < 30 |
Height Or Height Range: | Healthy - Mean (±SD): 171.8±10.3cm |
Gender: | Healthy - males and females | Sepsis - males and females |
Human Race: | Healthy - Caucasian (C) and African American (AA) | Sepsis - Caucasian (C) and African American (AA) |
Human Medications: | Healthy - None |
Human Prescription Otc: | Healthy - None |
Human Smoking Status: | Healthy - Non smokers |
Human Alcohol Drug Use: | Healthy - None |
Human Inclusion Criteria: | Healthy - non-smoking, non-obese with no known medical conditions that require chronic drug therapy | Sepsis - admitted to emergency department or intensive care unit. Clinical indices for severe sepsis or septic shock were met: confirmed or suspected infection, two or more criteria of systemic inflammatory response criteria, and hypoperfusion evidenced by either a systolic blood pressure of <90mmHg after 20 min of fluid resuscitation or a blood lactate level of at least 36 mg/dL. |
Species Group: | Human |
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Subject:
Subject ID: | SU000628 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | Healthy - Mean (±SD): 54.9±8.2 years | Sepsis - Mean (+SD): 54.7±17.3 years |
Weight Or Weight Range: | Healthy - Mean (+SD): 74.4±1.71 kg; BMI < 30 |
Height Or Height Range: | Healthy - Mean (±SD): 171.8±10.3cm |
Gender: | Healthy - males and females | Sepsis - males and females |
Human Race: | Healthy - Caucasian (C) and African American (AA) | Sepsis - Caucasian (C) and African American (AA) |
Human Medications: | Healthy - None |
Human Prescription Otc: | Healthy - None |
Human Smoking Status: | Healthy - Non smokers |
Human Alcohol Drug Use: | Healthy - None |
Human Inclusion Criteria: | Healthy - non-smoking, non-obese with no known medical conditions that require chronic drug therapy | Sepsis - admitted to emergency department or intensive care unit. Clinical indices for severe sepsis or septic shock were met: confirmed or suspected infection, two or more criteria of systemic inflammatory response criteria, and hypoperfusion evidenced by either a systolic blood pressure of <90mmHg after 20 min of fluid resuscitation or a blood lactate level of at least 36 mg/dL. |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
CCMB12WB | SA033435 | FL007385 | Whole Blood | Sample Type |
CCMB12WB | SA033435 | FL007385 | M | Gender |
CCMB12WB | SA033435 | FL007385 | C | Race |
Collection:
Collection ID: | CO000622 |
Collection Summary: | Healthy - Direct venipuncture collection of WB and Serum from healthy controls | Sepsis - Indwelling arterial or venous catheter |
Collection Protocol Comments: | Healthy - Fasting (12h) blood samples (serum and whole blood) were collected between 8:30-9:30am by single direct venipuncture. | Sepsis - Serum generated via indwelling catheters as part of clinical trial (NCT 00372502) |
Sample Type: | Healthy - Blood: Whole Blood and Serum | Sepsis - Blood serum |
Collection Method: | Healthy - Direct venipuncture | Sepsis - indwelling catheters |
Collection Location: | Healthy - Michigan Clinical Research Unit | Sepsis - University of Mississippi |
Collection Frequency: | Healthy - 1 | Sepsis - 1 |
Collection Time: | Healthy - Fasting, between 0830-0930 | Sepsis - N/A |
Storage Conditions: | Healthy - Serum: Initial RT then -80°C following processing (within 2h) Whole blood: Initial 0°C then -80°C following processing (within 10mins) | Sepsis - Initial RT then -80°C following processing (within 2h) |
Storage Vials: | Healthy - SST: Becton-Dickinson vacutainer Serum Separator tubes. WB: Becton-Dickinson vacutainer Sodium Heparin tubes | Sepsis - SST: Becton-Dickinson vacutainer Serum Separator tubes |
Collection Tube Temp: | Healthy - RT | Sepsis - RT |
Additives: | Healthy - Serum: none. Whole blood: Sodium Heparin | Sepsis - none |
Blood Serum Or Plasma: | Healthy - Whole blood and Serum | Sepsis - Serum |
Treatment:
Treatment ID: | TR000642 |
Treatment Summary: | No treatment |
Sample Preparation:
Sampleprep ID: | SP000635 |
Sampleprep Summary: | Methanol:cholorform extraction, filtration |
Sampleprep Protocol Comments: | Lipid fractions saved, stored at -20°C |
Processing Method: | Methanol:Chloroform Extraction, filtration |
Processing Storage Conditions: | 0-4°C |
Extraction Method: | Methanol:chloroform extraction |
Extract Enrichment: | Ultra-filtration to remove residual protein |
Extract Cleanup: | N/A |
Extract Storage: | -80°C |
Sample Resuspension: | 500 uL Deuterium Oxide (D2O) |
Sample Spiking: | DSS-IS2 (Chenomx, Inc) |
Analysis:
MB Sample ID: | SA033435 |
Analysis ID: | AN000926 |
Laboratory Name: | University of Michigan Biochemical NMR Core Laboratory |
Analysis Type: | NMR |
Acquisition Date: | 5/29/2013, 3/17/2014, 3/19/2014, 3/20/2014,7/29/2014, 8/28/2014 |
Acquisition Parameters File: | metnoesy |
Software Version: | VNMRJ 3.2 |
Operator Name: | Michael Finkel, Julie Trexel |
Data Format: | .fid |
Num Factors: | 6 |
Num Metabolites: | 53 |
Units: | uM |
NMR:
NMR ID: | NM000103 |
Analysis ID: | AN000926 |
Instrument Name: | Agilent 500/54 Premium Shielded VNMRS system |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D 1H |
Standard Concentration: | 10% (~0.5mM) |
Spectrometer Frequency: | 500 MHz |
NMR Probe: | ONE-Probe |
NMR Solvent: | D2O |
NMR Tube Size: | 5mm |
Shimming Method: | Auto shim (gradient shimming), manual when necessary. |
Pulse Sequence: | 1D-NOESY |
Water Suppression: | saturation at 80 Hz induced field strength |
Pulse Width: | 5.5ms |
Offset Frequency: | around -178Hz |
Chemical Shift Ref Cpd: | DSS-d6 (whole blood), Formate (serum) |
Temperature: | 25°C |
Baseline Correction Method: | manual |
Chemical Shift Ref Std: | DSS-d6 (whole blood), Formate (serum) |
Binned Increment: | N/A |
Binned Data Protocol File: | N A |
Binned Data Excluded Range: | N/A |