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MB Sample ID: SA034101
| Local Sample ID: | 18225 |
| Subject ID: | SU000638 |
| Subject Type: | Humans |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000638 |
| Subject Type: | Humans |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 18225 | SA034101 | FL007471 | Case control | Diagnosis |
| 18225 | SA034101 | FL007471 | - | RACE |
Collection:
| Collection ID: | CO000632 |
| Collection Summary: | Urine from ancestry typed AA and EA prostate cancer patients were used for this analysis. Urine specimens were spun and the supernatant was stored at −70°C until analysis. |
| Sample Type: | Urine |
Treatment:
| Treatment ID: | TR000652 |
| Treatment Summary: | NA |
Sample Preparation:
| Sampleprep ID: | SP000645 |
| Sampleprep Summary: | Urine supernatants were diluted with methanol containing spiked labeled internal standards (d3-sarcosine and d3-alanine), and extracted overnight at 4°C, using 1:1 molar ratio of water/chloroform at room temperature, with constant shaking. The aqueous methanolic layer was recovered, dried and azeotroped twice by adding 100 mL dimethylformamide (DMF), mixed and dried using a speedvac for 30 minutes. Following this, 100 mL of DMF and N-methyl-N-tert-butylmethylsilyltrifluoroacetamide (MtBSTFA) + 1% t-butyl-dimethylchlorosilane were added and incubated at 60°C for 1 hr. The samples were resuspended in ethyl acetate and injected into a GC-MS. |
Combined analysis:
| Analysis ID | AN000944 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent |
| Column | Agilent DB5-MS (15m) |
| MS Type | ESI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent |
| Ion Mode | POSITIVE |
| Units | Area |
Chromatography:
| Chromatography ID: | CH000672 |
| Chromatography Summary: | During the course of the run, temperature was ramped from 40° to 300° C in a 16 minute period. |
| Instrument Name: | Agilent |
| Column Name: | Agilent DB5-MS (15m) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000840 |
| Analysis ID: | AN000944 |
| Instrument Name: | Agilent |
| Instrument Type: | Single quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
