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MB Sample ID: SA034139
Local Sample ID: | 18018 |
Subject ID: | SU000638 |
Subject Type: | Humans |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000638 |
Subject Type: | Humans |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
18018 | SA034139 | FL007473 | Prostate cancer | Diagnosis |
18018 | SA034139 | FL007473 | Caucasian | RACE |
Collection:
Collection ID: | CO000632 |
Collection Summary: | Urine from ancestry typed AA and EA prostate cancer patients were used for this analysis. Urine specimens were spun and the supernatant was stored at −70°C until analysis. |
Sample Type: | Urine |
Treatment:
Treatment ID: | TR000652 |
Treatment Summary: | NA |
Sample Preparation:
Sampleprep ID: | SP000645 |
Sampleprep Summary: | Urine supernatants were diluted with methanol containing spiked labeled internal standards (d3-sarcosine and d3-alanine), and extracted overnight at 4°C, using 1:1 molar ratio of water/chloroform at room temperature, with constant shaking. The aqueous methanolic layer was recovered, dried and azeotroped twice by adding 100 mL dimethylformamide (DMF), mixed and dried using a speedvac for 30 minutes. Following this, 100 mL of DMF and N-methyl-N-tert-butylmethylsilyltrifluoroacetamide (MtBSTFA) + 1% t-butyl-dimethylchlorosilane were added and incubated at 60°C for 1 hr. The samples were resuspended in ethyl acetate and injected into a GC-MS. |
Combined analysis:
Analysis ID | AN000944 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent |
Column | Agilent DB5-MS (15m) |
MS Type | ESI |
MS instrument type | Single quadrupole |
MS instrument name | Agilent |
Ion Mode | POSITIVE |
Units | Area |
Chromatography:
Chromatography ID: | CH000672 |
Chromatography Summary: | During the course of the run, temperature was ramped from 40° to 300° C in a 16 minute period. |
Instrument Name: | Agilent |
Column Name: | Agilent DB5-MS (15m) |
Chromatography Type: | GC |
MS:
MS ID: | MS000840 |
Analysis ID: | AN000944 |
Instrument Name: | Agilent |
Instrument Type: | Single quadrupole |
MS Type: | ESI |
Ion Mode: | POSITIVE |