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MB Sample ID: SA035957
| Local Sample ID: | ms6103-19 |
| Subject ID: | SU000660 |
| Subject Type: | Rat |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000660 |
| Subject Type: | Rat |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| ms6103-19 | SA035957 | FL007544 | YCR1 kidney cortex | sample source |
| ms6103-19 | SA035957 | FL007544 | Transgenic Negative | group |
Collection:
| Collection ID: | CO000654 |
| Collection Summary: | The objective of the study is to identify Fumarase in hypertension. Samples are from Transgenic fumerase rats and WT littermates, 13 weeks of age |
| Sample Type: | Kidney |
Treatment:
| Treatment ID: | TR000674 |
| Treatment Summary: | We will analyze kidney tissue extract and urine samples from SS rats and the newly generated SS.Fh1+ transgenic rats. The SS.Fh1+ rat was generated using a Sleeping Beauty transposon-mediated transgenic technique. Overexpression of fumarase has been confirmed in SS.Fh1+ rats. Preliminary study indicated that the development of salt-induced hypertension was altered in SS.Fh1+ rats compared to wild-type littermate SS rats. We will analyze kidney tissue extract and urine samples collected from rats maintained on a 0.4% NaCl diet or switched to a 4% NaCl diet for 7 days. The dietary protocol has been used in numerous studies to examine mechanisms underlying salt sensitivity including early responses (7 days) to salt in the SS model. In total, 64 rat samples (two rat strains, two salt levels, and 8 rats per condition) will be analyzed. |
Sample Preparation:
| Sampleprep ID: | SP000667 |
| Sampleprep Summary: | Rat urine and kidney tissue extract (outer medulla and cortex) TCA concentrations. Urine TCA concentrations in uM and tissue in nmol/vial |
Combined analysis:
| Analysis ID | AN000969 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890B |
| Column | Agilent HP5-MS (30m × 0.25mm, 0.25 um) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5975C |
| Ion Mode | POSITIVE |
| Units | urine uM and Tissue nnol/vial |
Chromatography:
| Chromatography ID: | CH000694 |
| Instrument Name: | Agilent 7890B |
| Column Name: | Agilent HP5-MS (30m × 0.25mm, 0.25 um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000864 |
| Analysis ID: | AN000969 |
| Instrument Name: | Agilent 5975C |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
