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MB Sample ID: SA036032
Local Sample ID: | 2 |
Subject ID: | SU000663 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000663 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
2 | SA036032 | FL007597 | 11/26/13 | Date of Study |
2 | SA036032 | FL007597 | post-program | Time |
2 | SA036032 | FL007597 | Overweight/Obese adolescents 48 week excersise program | Study Group |
Collection:
Collection ID: | CO000657 |
Collection Summary: | Male and female American Indians 11-21 years old are being recruited from the Choctaw Nation Health Service Area of southeast Oklahoma. We use a collaborative approach as recently described by our American Indian Programs Coordinator (12). The primary characteristics of the three study groups are shown in Table 1. Group 1 is obese (Body mass index, BMI, ≥95th percentile for age- and sex-specific norms based on growth charts from the Centers for Disease Control), with pubertal maturation level ≥Tanner Stage 2, family history of T2D, and recent history of low physical activity. Low physical activity, defined as attaining <30 minutes of structured moderate-to-vigorous intensity exercise on 3 days/week over the preceding 3 months, is confirmed through questionnaires and use of an accelerometer. Groups 2 and 3 are both classified as normal weight (BMI greater than 20th and less than 85th percentile) with maturation level ≥Tanner Stage 2. Group 2 will have low physical activity (same as Group 1), while Group 3 will have high physical activity, defined as >30 minutes of structured moderate-to-vigorous intensity exercise on >3 days/week in the preceding 3 months. Additional eligibility criteria are: lack of diabetes or other potentially confounding metabolic disorders, able to safely exercise, willingness to complete the testing and participation schedule, and not on medications known to impact the stated outcomes. A medical history and physical exam is performed to assure suitability for the study during the initial screening. |
Sample Type: | Blood |
Treatment:
Treatment ID: | TR000677 |
Treatment Summary: | Only Group 1 participants continue on to the exercise training program, which lasts 48 weeks. Follow-up measurements of the clinical and functional tests are performed at 16, 32, and 48 weeks; values at 16 weeks will be used for the current proposal to assure adequate sample size. Exercise sessions are performed at the Choctaw Wellness Centers, where fitness professionals provide instruction and supervision. Participants receive incentives for completing 20-60 minutes of moderate-to-vigorous physical activity (MVPA) on 3 days per week. MVPA is defined as exercise that elicits a heart rate ≥ 70% of peak heart rate. Several types of exercise can be selected, including walking, running, cycling, aerobic dance or other activities. To promote healthy behavior within the community and foster social support, all participants are invited to exercise with family members or friends if they choose. Exercise intensity is monitored by recording heart rate with a chest strap transmitter that outputs to a computer for quantification and data storage. Training sessions are also recorded in an exercise log by each participant and confirmed by fitness center staff. |
Sample Preparation:
Sampleprep ID: | SP000670 |
Sampleprep Summary: | Part 1: Longer-term exercise training study: Samples #1-133. Overweight/Obese adolescents (IDs 434-001 through 434-132) enrolled into a 48-week exercise program (3 days per week). Samples submitted are from the pre-training (Baseline) and the end of the first 16 weeks (Post-training) times. Compliance with the exercise program varies widely among participants. An additional set of normal weight reference group adolescents (IDs 434-301 through 434-308) are included. Note that 15 OW/OB participants in the exercise group and all 8 NW reference group participants have only a single baseline sample. All samples were collected following overnight fast into EDTA (lavender top) glass tubes for preparation of plasma, kept on ice 20 minutes, spun, aliquoted and then kept frozen. None have been previously used. Part 2: Short-term exercise training study: Samples with ID 11xxx. Overweight/Obese adolescents completed a short-term exercise program, consisting of 10 sessions over 5 weeks. Samples were collected following overnight fast, before (Baseline) and after (Post-training) the training program. These samples are serum since plasma was used for other analyses. All tubes are marked with a unique ID number (shown in list below) in black marker. Additional sample details on the labels are listed in the accompanying Excel spreadsheet. |
Combined analysis:
Analysis ID | AN000972 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 1290 Infinity |
Column | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Agilent 6460 QQQ |
Ion Mode | NEGATIVE |
Units | uM |
Chromatography:
Chromatography ID: | CH000697 |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000867 |
Analysis ID: | AN000972 |
Instrument Name: | Agilent 6460 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | NEGATIVE |