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MB Sample ID: SA042833
Local Sample ID: | CMC2029-0 |
Subject ID: | SU000808 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 39-93 years |
Weight Or Weight Range: | 55-130 KG |
Gender: | males and females |
Human Race: | Caucasian (C) and African American (AA) |
Human Inclusion Criteria: | Suspicion of or confirmed infection; two or more systemic infmammatory response syndrome criteria; hypotension requiring vasopressors despite volume resuscitation; a cumulative vasopressor index of 3 or more after 4 hours of administration; and a SOFA score of at least 5. |
Human Exclusion Criteria: | Age less than 18; greater than 16h after septic shock recognition; a Do Not Resuscitate order; or known inborn error of metaboloism. |
Species Group: | Human |
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Subject:
Subject ID: | SU000808 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 39-93 years |
Weight Or Weight Range: | 55-130 KG |
Gender: | males and females |
Human Race: | Caucasian (C) and African American (AA) |
Human Inclusion Criteria: | Suspicion of or confirmed infection; two or more systemic infmammatory response syndrome criteria; hypotension requiring vasopressors despite volume resuscitation; a cumulative vasopressor index of 3 or more after 4 hours of administration; and a SOFA score of at least 5. |
Human Exclusion Criteria: | Age less than 18; greater than 16h after septic shock recognition; a Do Not Resuscitate order; or known inborn error of metaboloism. |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
CMC2029-0 | SA042833 | FL008919 | Carnitine | Treatment |
Collection:
Collection ID: | CO000802 |
Collection Summary: | Serum collected via existing intravenous or arterial catheter |
Collection Protocol Comments: | T0: collected before carnitine or placebo administration, T24: collected 24h after administration of L-carnitine or placebo (±4h), T48: collected 48h after administration of L-carnitine or placebo (±4h) |
Sample Type: | Blood serum |
Collection Method: | via existing intravenous or arterial catheter |
Collection Frequency: | 1/timepoint, 3 collections/48h |
Collection Time: | T0: before administration, T24: 24h after administration (±4h), T48: 48h after administration (±4h) |
Volumeoramount Collected: | 10mL |
Storage Conditions: | Initial RT then -80°C following processing (within 2h of collection) |
Collection Vials: | SST: Becton-Dickinson 10mL vacutainer Serum Separator tubes |
Storage Vials: | Sterile 1mL cryovials |
Collection Tube Temp: | RT |
Additives: | None |
Blood Serum Or Plasma: | Serum |
Treatment:
Treatment ID: | TR000822 |
Treatment Summary: | Intravenous carnitine |
Treatment Protocol Comments: | Non-treatment group: equivalent volume of saline placebo |
Treatment Compound: | L-carnitine |
Treatment Route: | 4g bolus followed by an 8g infusion over 12h |
Treatment Dose: | 12g |
Treatment Doseduration: | 12h (following initial 4g bolus) |
Sample Preparation:
Sampleprep ID: | SP000815 |
Sampleprep Summary: | Methanol:cholorform extraction, filtration |
Sampleprep Protocol Comments: | Lipid fractions saved, stored at -20°C |
Processing Method: | Methanol:Chloroform Extraction, filtration |
Processing Storage Conditions: | 0-4°C |
Extraction Method: | Methanol:chloroform extraction |
Extract Enrichment: | Ultra-filtration (3kDa filters) to remove residual protein |
Extract Cleanup: | N/A |
Extract Storage: | -80 C |
Sample Resuspension: | =500 uL Deuterium Oxide (D2O) |
Sample Spiking: | CaFormate 12mM |
Analysis:
MB Sample ID: | SA042833 |
Analysis ID: | AN001244 |
Laboratory Name: | University of Michigan Biochemical NMR Core Laboratory |
Analysis Type: | NMR |
Software Version: | VNMRJ 3.2 |
Operator Name: | Michael Finkel, Julie Trexel |
Detector Type: | NMR |
Data Format: | .FID |
Num Factors: | 2 |
Num Metabolites: | 38 |
Units: | µM |
NMR:
NMR ID: | NM000105 |
Analysis ID: | AN001244 |
Instrument Name: | Agilent 500/54 Premium Shielded VNMRS system |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D 1H |
NMR Comments: | Experiment dates: 11/7/2013, 11/11/2013, 11/19/2013 |
Standard Concentration: | 10% (~0.5mM) |
Spectrometer Frequency: | 500 MHz |
NMR Probe: | ONE-Probe |
NMR Solvent: | D2O |
NMR Tube Size: | 5mm |
Shimming Method: | Auto shim (gradient shimming) |
Pulse Sequence: | 1D-NOESY |
Water Suppression: | saturation at 80 Hz induced field strength |
Pulse Width: | 5.5ms |
Power Level: | around -178Hz |
Chemical Shift Ref Cpd: | Formate |
Temperature: | 25°C |
Number Of Scans: | 32 |
Baseline Correction Method: | manual |
Chemical Shift Ref Std: | Calcium Formate |