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MB Sample ID: SA043035
| Local Sample ID: | Intestine_Model1 |
| Subject ID: | SU000810 |
| Subject Type: | Animals |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000810 |
| Subject Type: | Animals |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Intestine_Model1 | SA043035 | FL008934 | Intestine | Tissue |
| Intestine_Model1 | SA043035 | FL008934 | Carcinoma | Treatment |
Collection:
| Collection ID: | CO000804 |
| Collection Summary: | Briefly, tissues of intestines, kidneys and spleens weighting about 200 mg, 200 mg and 110 mg were weighted, homogenized with an icy cold solvent (the ratio of tissue weight to 50 % acetonitrile volume was 1: 5, w/v), vortexed, and centrifuged for 10 min at 12,000 g and 4 °C. The supernatant was transferred into fresh Eppendorf tube and was then frozen and lyophilized to dryness on a vacuum concentrator. The dried samples were stored at -80 °C until use. |
| Sample Type: | Intestine |
Treatment:
| Treatment ID: | TR000824 |
| Treatment Summary: | The animals were maintained under controlled conditions (22°C, 12h/12h 98 dark/light cycle). At the 15th week, there were 15 surviving mice in control group, 17 in model group, 14 in JHC group and 10 in CNC group, respectively. In this work, rats were divided into control group, carcinoma group, and two drug groups of JHC and CNC. The acronyms JHC means the water-soluble fraction of Camellia nitidissima Chi. The acronyms CNC means the butanol fraction of Camellia nitidissima Chi. |
Sample Preparation:
| Sampleprep ID: | SP000817 |
| Sampleprep Summary: | For NMR measurements, samples were dissolved in 550 µL 99.8% deuteroxide (D2O) phosphate buffer (0.2 M, pH = 7.4) containing 0.05% (w/v) sodium 3-(trimethylsilyl) propionate-2, 2, 3, 3-d4 (TSP). After vortexing and centrifugation, the supernatant was then transferred to a 5 mm NMR tube for 1H NMR analysis. |
Analysis:
| MB Sample ID: | SA043035 |
| Analysis ID: | AN001238 |
| Analysis Type: | NMR |
| Num Factors: | 12 |
NMR:
| NMR ID: | NM000106 |
| Analysis ID: | AN001238 |
| Instrument Name: | Bruker |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Spectrometer Frequency: | 500 MHz |
