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MB Sample ID: SA045807
Local Sample ID: | S00021740 |
Subject ID: | SU000845 |
Subject Type: | HUMAN |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000845 |
Subject Type: | HUMAN |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
S00021740 | SA045807 | FL009111 | shACADMscr | Transduced with |
Collection:
Collection ID: | CO000839 |
Collection Summary: | - |
Sample Type: | Cultured cells |
Treatment:
Treatment ID: | TR000859 |
Treatment Summary: | - |
Sample Preparation:
Sampleprep ID: | SP000852 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | A004_-_Shotgun_Lipidomics.pdf |
Combined analysis:
Analysis ID | AN001300 | AN001301 | AN001302 |
---|---|---|---|
Analysis type | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | Reversed phase |
Chromatography system | Shimadzu CTO-20A Nexera X2 | Shimadzu CTO-20A Nexera X2 | Agilent |
Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Xbridge BEH C18 (50 x 1mm,3.5um) |
MS Type | ESI | ESI | ESI |
MS instrument type | Triple TOF | Triple TOF | Triple quadrupole |
MS instrument name | ABI Sciex 5600+ TripleTOF | ABI Sciex 5600+ TripleTOF | Agilent 6490A QQQ |
Ion Mode | NEGATIVE | POSITIVE | POSITIVE |
Units | peak area normalized | peak area normalized | pmol/ug protein |
Chromatography:
Chromatography ID: | CH000910 |
Instrument Name: | Shimadzu CTO-20A Nexera X2 |
Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
Column Temperature: | 55 |
Flow Rate: | 0.400uL/min |
Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH000911 |
Instrument Name: | Agilent |
Column Name: | Waters Xbridge BEH C18 (50 x 1mm,3.5um) |
Column Temperature: | 40 |
Flow Rate: | 0.250 mL/min |
Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001193 |
Analysis ID: | AN001300 |
Instrument Name: | ABI Sciex 5600+ TripleTOF |
Instrument Type: | Triple TOF |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
MS ID: | MS001194 |
Analysis ID: | AN001301 |
Instrument Name: | ABI Sciex 5600+ TripleTOF |
Instrument Type: | Triple TOF |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
MS ID: | MS001195 |
Analysis ID: | AN001302 |
Instrument Name: | Agilent 6490A QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Analysis Protocol File: | A008_-_Acyl_Carnitines.pdf |