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MB Sample ID: SA051924
Local Sample ID: | EL-NYMC-116-Run 5 |
Subject ID: | SU000925 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000925 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
EL-NYMC-116-Run 5 | SA051924 | FL009957 | Early Localized Lyme | Disease Status |
Collection:
Collection ID: | CO000919 |
Collection Summary: | Sera from early LD patients were collected pretreatment at the initial visit to the clinic. Healthy control serum donors were from endemic and nonendemic regions for LD. |
Sample Type: | Blood |
Treatment:
Treatment ID: | TR000939 |
Treatment Summary: | N/A |
Sample Preparation:
Sampleprep ID: | SP000932 |
Sampleprep Summary: | Small molecule metabolites were extracted from aliquots (20 μL) of sera with 75% (final vol) HPLC grade methanol. An aliquot equivalent to 5 μL of serum was analyzed by LC-MS. |
Sampleprep Protocol Comments: | doi:10.1038/nprot.2011.335 |
Combined analysis:
Analysis ID | AN001450 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 1200 |
Column | Poroshell 120,EC-C8(100 x 2.1mm,2.5um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Agilent 6520 QTOF |
Ion Mode | POSITIVE |
Units | peak area |
Chromatography:
Chromatography ID: | CH001019 |
Instrument Name: | Agilent 1200 |
Column Name: | Poroshell 120,EC-C8(100 x 2.1mm,2.5um) |
Flow Gradient: | 0-100% non-linear gradient |
Flow Rate: | 0.35 ml/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% methanol; 0.1% formic acid |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001340 |
Analysis ID: | AN001450 |
Instrument Name: | Agilent 6520 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Capillary Voltage: | 2000 V |
Dry Gas Flow: | 8 l/min |
Dry Gas Temp: | 300 |
Fragment Voltage: | 120 V |
Nebulizer: | 45 psi |
Octpole Voltage: | 750 V |
Scanning Cycle: | 666.7 spectra/s |
Scanning Range: | 115 - 1500 Da |
Skimmer Voltage: | 60 V |