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MB Sample ID: SA052169
| Local Sample ID: | HNC-CO-21-Run 5 |
| Subject ID: | SU000925 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000925 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| HNC-CO-21-Run 5 | SA052169 | FL009961 | Healthy Non-endemic control | Disease Status |
Collection:
| Collection ID: | CO000919 |
| Collection Summary: | Sera from early LD patients were collected pretreatment at the initial visit to the clinic. Healthy control serum donors were from endemic and nonendemic regions for LD. |
| Sample Type: | Blood |
Treatment:
| Treatment ID: | TR000939 |
| Treatment Summary: | N/A |
Sample Preparation:
| Sampleprep ID: | SP000932 |
| Sampleprep Summary: | Small molecule metabolites were extracted from aliquots (20 μL) of sera with 75% (final vol) HPLC grade methanol. An aliquot equivalent to 5 μL of serum was analyzed by LC-MS. |
| Sampleprep Protocol Comments: | doi:10.1038/nprot.2011.335 |
Combined analysis:
| Analysis ID | AN001450 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1200 |
| Column | Poroshell 120,EC-C8(100 x 2.1mm,2.5um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6520 QTOF |
| Ion Mode | POSITIVE |
| Units | peak area |
Chromatography:
| Chromatography ID: | CH001019 |
| Instrument Name: | Agilent 1200 |
| Column Name: | Poroshell 120,EC-C8(100 x 2.1mm,2.5um) |
| Flow Gradient: | 0-100% non-linear gradient |
| Flow Rate: | 0.35 ml/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% methanol; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001340 |
| Analysis ID: | AN001450 |
| Instrument Name: | Agilent 6520 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Capillary Voltage: | 2000 V |
| Dry Gas Flow: | 8 l/min |
| Dry Gas Temp: | 300 |
| Fragment Voltage: | 120 V |
| Nebulizer: | 45 psi |
| Octpole Voltage: | 750 V |
| Scanning Cycle: | 666.7 spectra/s |
| Scanning Range: | 115 - 1500 Da |
| Skimmer Voltage: | 60 V |
