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MB Sample ID: SA055559

Local Sample ID:Inactivated-0100_A_15
Subject ID:SU000971
Subject Type:Bacteria
Subject Species:Mycobacterium tuberculosis H37Rv
Taxonomy ID:83332

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Subject:

Subject ID:SU000971
Subject Type:Bacteria
Subject Species:Mycobacterium tuberculosis H37Rv
Taxonomy ID:83332

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
Inactivated-0100_A_15SA055559FL01018315timepoint (min)
Inactivated-0100_A_15SA055559FL010183Inactiveprotein prep

Collection:

Collection ID:CO000965
Collection Summary:Rv0100 from M. tuberculosis H37Rv was expressed in E. coli cells and purified.
Sample Type:Cultured cells

Treatment:

Treatment ID:TR000985
Treatment Summary:Protein was incubated with whole-cell lysate in either Active form in Inactive (heat-inactivated).

Sample Preparation:

Sampleprep ID:SP000978
Sampleprep Summary:Protein was incubated with whole-cell lysate in either Active form or Inactive (heat-inactivated).

Combined analysis:

Analysis ID AN001527 AN001528
Analysis type MS MS
Chromatography type Normal phase Normal phase
Chromatography system Agilent 1290 Agilent 1290
Column Microsolv Diamond hydride (150mm,4um) Microsolv Diamond hydride (150mm,4um)
MS Type ESI ESI
MS instrument type TOF TOF
MS instrument name Agilent 6220 TOF Agilent 6220 TOF
Ion Mode POSITIVE NEGATIVE
Units peak counts peak counts

Chromatography:

Chromatography ID:CH001076
Chromatography Summary:Aqueous normal phase diamond hydride
Instrument Name:Agilent 1290
Column Name:Microsolv Diamond hydride (150mm,4um)
Chromatography Type:Normal phase

MS:

MS ID:MS001407
Analysis ID:AN001527
Instrument Name:Agilent 6220 TOF
Instrument Type:TOF
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS001408
Analysis ID:AN001528
Instrument Name:Agilent 6220 TOF
Instrument Type:TOF
MS Type:ESI
Ion Mode:NEGATIVE
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