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MB Sample ID: SA055663

Local Sample ID:Inactivated-2553_C_30
Subject ID:SU000974
Subject Type:Bacteria
Subject Species:Mycobacterium tuberculosis H37Rv
Taxonomy ID:83332

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Subject:

Subject ID:SU000974
Subject Type:Bacteria
Subject Species:Mycobacterium tuberculosis H37Rv
Taxonomy ID:83332

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
Inactivated-2553_C_30SA055663FL01019530timepoint (min)
Inactivated-2553_C_30SA055663FL010195Inactiveprotein prep

Collection:

Collection ID:CO000968
Collection Summary:Rv2553c from M. tuberculosis H37Rv was expressed in E. coli cells and purified.
Sample Type:Cultured cells

Treatment:

Treatment ID:TR000988
Treatment Summary:Protein was incubated with whole-cell lysate in either Active form in Inactive (heat-inactivated). Protein was incubated with whole-cell lysate in either Active form in Inactive (heat-inactivated).

Sample Preparation:

Sampleprep ID:SP000981
Sampleprep Summary:Protein was incubated with whole-cell lysate in either Active form or Inactive (heat-inactivated). Protein was incubated with whole-cell lysate in either Active form or Inactive(heat-inactivated).

Combined analysis:

Analysis ID AN001532 AN001533
Analysis type MS MS
Chromatography type Normal phase Normal phase
Chromatography system Agilent 1290 Agilent 1290
Column Microsolv Diamond hydride (150mm,4um) Microsolv Diamond hydride (150mm,4um)
MS Type ESI ESI
MS instrument type TOF TOF
MS instrument name Agilent 6220 TOF Agilent 6220 TOF
Ion Mode POSITIVE NEGATIVE
Units peak counts peak counts

Chromatography:

Chromatography ID:CH001079
Chromatography Summary:Aqueous normal phase diamond hydride
Instrument Name:Agilent 1290
Column Name:Microsolv Diamond hydride (150mm,4um)
Chromatography Type:Normal phase

MS:

MS ID:MS001412
Analysis ID:AN001532
Instrument Name:Agilent 6220 TOF
Instrument Type:TOF
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS001413
Analysis ID:AN001533
Instrument Name:Agilent 6220 TOF
Instrument Type:TOF
MS Type:ESI
Ion Mode:NEGATIVE
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