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MB Sample ID: SA058373

Local Sample ID:philic-21mar17-010-r002
Subject ID:SU001010
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

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Subject:

Subject ID:SU001010
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
philic-21mar17-010-r002SA058373FL010429Group 2Group

Collection:

Collection ID:CO001004
Collection Summary:Samples were collected by cardiac puncture and plasma was collected following standard centrifugation steps. Immediately following the samples were separated into 500 uL aliquots in 600 uL tubes and frozen in liquid nitrogen.
Sample Type:Blood (plasma)

Treatment:

Treatment ID:TR001024
Treatment Summary:We administered normal, high glucose, aspartame, and acesulfame potassium diets to rats for 3 weeks, followed by a plasma collection through cardiac puncture and metabolic analysis (Group 1-4 samples). We also treated the gut microbiota with in rats with the same diets plus bacitracin/streptomycin to observe how alterations of the microbiome influence the plasma metabolic profile in these animals (Groups 5-8). The samples here contain the fructose diet group (Group 9). We also treated the gut microbiota with in rats with the same diets plus bacitracin/streptomycin to observe how alterations of the microbiome influence the plasma metabolic profile in these animals (Groups 5-8). The samples here contain the fructose diet group with antibiotic (Group 10). For the gut microbiota experiment, during the last 10 days of the diet subsets of all groups will have bacitracin and streptomycin (B/S) provided in their drinking water (0.5g/250 mL). The resulting data will give us insights into the influence of high sugar and artificial sweetener diets on homeostatic metabolic processes and dive into the symbiotic relationship of the gut microbiome with this process. Group1 = normal diet Group2 = high glucose diet Group3 = aspartame diet Group4 = acesulfame potassium diet Group5 = rat gut microbiota normal diet + antibotics Group6 = rat gut microbiota high glucose diet + antibotics Group7 = rat gut microbiota aspartame diet + antibotics Group8 = rat gut acesulfame potassium diet + antibotics Group9 = fructose diet Group10 = rat gut fructose diet + antibotics

Sample Preparation:

Sampleprep ID:SP001017
Sampleprep Summary:large scale profiling

Combined analysis:

Analysis ID AN001588 AN001589 AN001590 AN001591
Analysis type MS MS MS MS
Chromatography type HILIC HILIC Reversed phase Reversed phase
Chromatography system Agilent 1290 Infinity Agilent 1290 Infinity Agilent 1290 Infinity Agilent 1290 Infinity
Column Waters Acquity BEH Amide (150 x 2.1mm,1.7um) Waters Acquity BEH Amide (150 x 2.1mm,1.7um) Waters Acquity HSS C18 (150 x 2.1mm,1.8um) Waters Acquity HSS C18 (150 x 2.1mm,1.8um)
MS Type ESI ESI ESI ESI
MS instrument type QTOF QTOF QTOF QTOF
MS instrument name Agilent 6550 QTOF Agilent 6550 QTOF Agilent 6550 QTOF Agilent 6550 QTOF
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units intensity intensity intensity intensity

Chromatography:

Chromatography ID:CH001115
Instrument Name:Agilent 1290 Infinity
Column Name:Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
Chromatography Type:HILIC
  
Chromatography ID:CH001116
Instrument Name:Agilent 1290 Infinity
Column Name:Waters Acquity HSS C18 (150 x 2.1mm,1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS001466
Analysis ID:AN001588
Instrument Name:Agilent 6550 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS001467
Analysis ID:AN001589
Instrument Name:Agilent 6550 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS001468
Analysis ID:AN001590
Instrument Name:Agilent 6550 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS001469
Analysis ID:AN001591
Instrument Name:Agilent 6550 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
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