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MB Sample ID: SA058651
| Local Sample ID: | S-462 |
| Subject ID: | SU001012 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Human Ethnicity: | Yoruba |
| Human Inclusion Criteria: | apparent health, no recent treatment for any disease, no recent use of antibiotics, antimalarial or other drugs |
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Subject:
| Subject ID: | SU001012 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Human Ethnicity: | Yoruba |
| Human Inclusion Criteria: | apparent health, no recent treatment for any disease, no recent use of antibiotics, antimalarial or other drugs |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| S-462 | SA058651 | FL010441 | INFECTED | Status |
| S-462 | SA058651 | FL010441 | INFECTION-ONLY | Stage |
Collection:
| Collection ID: | CO001006 |
| Collection Summary: | blood and urine samples collected from volunteers in the morning hours. Plasma was separated from blood. urine was examined for S. haematobium infection. Distended Bladder scans were carried out |
| Collection Protocol ID: | UI/EC/15/0019 |
| Sample Type: | Plasma;Urine |
Treatment:
| Treatment ID: | TR001026 |
| Treatment Summary: | Samples were categorised as infected, infected with pathologies, and controls. Infection with Schistosoma haematobium infection by examining urine under the microscopy to detect eggs and by amplifying Dra 1 region. Pathologies were determined ultrasonography |
Sample Preparation:
| Sampleprep ID: | SP001019 |
| Sampleprep Summary: | Both urine and plasma samples were prepared using chilled methanol:water mixture (4:1). |
Combined analysis:
| Analysis ID | AN001594 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Q-Exactive hybrid quadrupole orbitrap mass spectrometer |
| Column | Hypersil Gold |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE |
| Units | abundance of specific m/z (amu) |
Chromatography:
| Chromatography ID: | CH001119 |
| Chromatography Summary: | Both urine and plasma samples were prepared using chilled methanol:water mixture (4:1). Separation was achieved with 150mm x 2.1mm, 1.9μ Hypersil Gold column with a 5μl injection volume. Gradient elution was performed using 0.1% formic acid in water (A) and acetonitrile (B) as mobile phase after optimization. The column was washed between each sample for stability and to eliminate carry-over. The flow rate was 0.35 ml/min. MS acquisition was performed on the Q-Exactive hybrid quadrupole orbitrap mass spectrometer (ThermoFisher, MA, USA) operated in positive and negative electrospray ionization (ESI) modes. The scan range was 70 to 1000 m/z. |
| Instrument Name: | Q-Exactive hybrid quadrupole orbitrap mass spectrometer |
| Column Name: | Hypersil Gold |
| Flow Rate: | 0.35ml/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001472 |
| Analysis ID: | AN001594 |
| Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
