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MB Sample ID: SA062947
| Local Sample ID: | nhilic-15aug17-114 |
| Subject ID: | SU001041 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001041 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| nhilic-15aug17-114 | SA062947 | FL010792 | 4 | hour |
| nhilic-15aug17-114 | SA062947 | FL010792 | day4 | day |
| nhilic-15aug17-114 | SA062947 | FL010792 | CR -20 storage | Group |
Collection:
| Collection ID: | CO001035 |
| Collection Summary: | Serial urine samples were collected at each void (approximately every 3 hours) from subjects during a 6-day inpatient protocol. The total volume of each sample was measured, and then 5 mL was aliquoted into a 7 mL tube and delivered on ice to the processing lab, where the samples were then stored at -80 degrees. At the end of the study, samples were transported (~2 blocks) from the processing lab to our -80 freezer for storage. |
| Sample Type: | Urine |
Treatment:
| Treatment ID: | TR001055 |
| Treatment Summary: | This subject (subject code: 3635A) spent 6 days in the lab: 3 baseline days where the subject slept for 8 hours at night (at habitual times as determined during the screening period) and 16 hours of ambulatory wake in ambient light, followed by 50 hours of continuous wakefulness in which the subject was kept in a semi-recumbent position in bed under dim light and fed hourly isocaloric snacks (called a constant routine). In the study design, baseline and CR are used to group the 3 day baseline days and constant routine days. |
Sample Preparation:
| Sampleprep ID: | SP001048 |
| Sampleprep Summary: | large scale profiling |
Combined analysis:
| Analysis ID | AN001637 | AN001638 | AN001639 | AN001640 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | HILIC | HILIC | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity | Agilent 1290 Infinity | Agilent 1290 Infinity |
| Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) | Waters Acquity HSS C18 (150 x 2.1mm,1.8um) | Waters Acquity HSS C18 (150 x 2.1mm,1.8um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | QTOF | QTOF | QTOF | QTOF |
| MS instrument name | Agilent 6550 QTOF | Agilent 6550 QTOF | Agilent 6550 QTOF | Agilent 6550 QTOF |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | intensity | intensity | intensity | intensity |
Chromatography:
| Chromatography ID: | CH001153 |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH001154 |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters Acquity HSS C18 (150 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001513 |
| Analysis ID: | AN001637 |
| Instrument Name: | Agilent 6550 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS001514 |
| Analysis ID: | AN001638 |
| Instrument Name: | Agilent 6550 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| MS ID: | MS001515 |
| Analysis ID: | AN001639 |
| Instrument Name: | Agilent 6550 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS001516 |
| Analysis ID: | AN001640 |
| Instrument Name: | Agilent 6550 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
