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MB Sample ID: SA072486
Local Sample ID: | MMV1432165-20170130 |
Subject ID: | SU001118 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | 3D7 |
Cell Counts: | 1x10^8 per technical replicate |
Species Group: | Plasmodium falciparum |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001118 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | 3D7 |
Cell Counts: | 1x10^8 per technical replicate |
Species Group: | Plasmodium falciparum |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
MMV1432165-20170130 | SA072486 | FL011366 | MMV1432165 | Drug |
MMV1432165-20170130 | SA072486 | FL011366 | 1070 nM | Treatment conc. |
Collection:
Collection ID: | CO001112 |
Collection Summary: | Aspiration of 4 mL media, transfer of sample (1 mL containing infected red blood cells) to 1.5 mL tubes |
Collection Protocol Filename: | Metabolite_Extraction_for_LCMS_2017_Llinas.pdf |
Sample Type: | Parasite |
Collection Method: | Aspiration of 4 mL media, transfer of sample (1 mL) to 1.5 mL tubes |
Collection Location: | Biological safety hood |
Collection Frequency: | once after 2.5 hours treatment |
Collection Duration: | Collection and extraction occurs in under 20 minutes per plate |
Volumeoramount Collected: | 1 mL containing 1x10^8 infected cells |
Storage Conditions: | On ice |
Collection Vials: | 1.5 mL eppendorf |
Storage Vials: | 1.5 mL eppendorf |
Treatment:
Treatment ID: | TR001132 |
Treatment Summary: | Infected Red Blood cells were exposed to Antimalarial Compounds, associated with sample names |
Treatment Protocol Filename: | Metabolite_Extraction_for_LCMS_2017_Llinas.pdf |
Treatment: | Drug Treatment |
Treatment Compound: | MMV1432165, MMV1451822, MMV1068987, MMV1490405, MMV1490397, MMV1449124, MMV1432711, MMV1454442, MMV1010248, MMV1319550, MMV1427995, MMV1431916, MMV011772 |
Treatment Route: | Addition to media |
Treatment Dose: | 10xIC50 |
Treatment Dosevolume: | 10xIC50 |
Treatment Doseduration: | 2.5 hours |
Treatment Vehicle: | Stocks kept in either DMSO or Water |
Cell Storage: | Temperature/Gas Mixture controlled incubator |
Cell Growth Container: | 6-well plate |
Cell Growth Config: | 5 mL per well, each with 1x10^8 cells |
Cell Media: | RPMI 1640 w/ Albumax, Gentamycin, Hypoxanthine, HEPES, Sodium Bicarbonate |
Cell Harvesting: | PBS Wash followed by 90% methanol extraction |
Sample Preparation:
Sampleprep ID: | SP001125 |
Sampleprep Summary: | Samples washed in PBS, spun down, aspirated, extracted in 90% methanol with labelled aspartate, then dried, resuspended in water labelled with chlorpropamide |
Sampleprep Protocol Filename: | Metabolite_Extraction_for_LCMS_2017_Llinas.pdf |
Processing Method: | Wash, Lysis, Drying, resuspension |
Processing Storage Conditions: | On ice |
Extraction Method: | 90% Methanol Lysis |
Extract Storage: | -80℃ |
Sample Resuspension: | 100 uL water with chlorpropamide |
Combined analysis:
Analysis ID | AN001756 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 |
Column | Phenomenex Synergi Hydro RP (100 x 2mm,2.5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Plus Orbitrap |
Ion Mode | NEGATIVE |
Units | Average Peak Area |
Chromatography:
Chromatography ID: | CH001240 |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Phenomenex Synergi Hydro RP (100 x 2mm,2.5um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001624 |
Analysis ID: | AN001756 |
Instrument Name: | Thermo Q Exactive Plus Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |