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MB Sample ID: SA072534
Local Sample ID: | MQ_38 |
Subject ID: | SU001120 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | Ndufs4, https://www.jax.org/strai n/02705 8 |
Age Or Age Range: | 45-50 days |
Gender: | Male |
Animal Animal Supplier: | Jackson Laboratory (ME, USA) |
Animal Light Cycle: | 12:12 h |
Animal Feed: | Rodent Breeder, Cat. #RM1845, LabChef, Nutritionhub |
Animal Water: | ad libitum |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001120 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | Ndufs4, https://www.jax.org/strai n/02705 8 |
Age Or Age Range: | 45-50 days |
Gender: | Male |
Animal Animal Supplier: | Jackson Laboratory (ME, USA) |
Animal Light Cycle: | 12:12 h |
Animal Feed: | Rodent Breeder, Cat. #RM1845, LabChef, Nutritionhub |
Animal Water: | ad libitum |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
MQ_38 | SA072534 | FL011377 | Ndufs4-/- | Genotype |
Collection:
Collection ID: | CO001114 |
Collection Summary: | Mice were euthanized between postnatal day (P) 45-50 via cervical dislocation at the same time of day (8:00-9:00 AM) after overnight (12-h) fasting. From both hind limbs, white muscle portions of quadriceps femoris, as well as soleus muscles were collected. Tissues were snap-frozen in liquid nitrogen (within 15 min post-mortem) and stored at -80°C until used. |
Sample Type: | Muscle |
Treatment:
Treatment ID: | TR001134 |
Treatment Summary: | The animals did not receive any treatment. |
Sample Preparation:
Sampleprep ID: | SP001127 |
Sampleprep Summary: | Muscle tissues were finely minced and homogenized in the presence of internal standards using a vibration mill. Metabolite extraction was achieved using a modified monophasic Bligh–Dyer extraction method with a solvent ratio of 3:1:1 (methanol:water:chloroform). |
Sampleprep Protocol Filename: | LC_quadriceps_sample_prep_protocol.pdf |
Combined analysis:
Analysis ID | AN001758 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 1200 |
Column | Agilent C18 Zorbax SB-AQ (150 x 2.1mm, 3.5um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Agilent 6410 QQQ |
Ion Mode | POSITIVE |
Units | Normalized peak area |
Chromatography:
Chromatography ID: | CH001242 |
Instrument Name: | Agilent 1200 |
Column Name: | Agilent C18 Zorbax SB-AQ (150 x 2.1mm, 3.5um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001626 |
Analysis ID: | AN001758 |
Instrument Name: | Agilent 6410 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | POSITIVE |