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MB Sample ID: SA072619
Local Sample ID: | MQ_29 |
Subject ID: | SU001122 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | Ndufs4, https://www.jax.org/strai n/02705 8 |
Age Or Age Range: | 45-50 days |
Gender: | Male |
Animal Animal Supplier: | Jackson Laboratory (ME, USA) |
Animal Light Cycle: | 12:12 h |
Animal Feed: | Rodent Breeder, Cat. #RM1845, LabChef, Nutritionhub |
Animal Water: | ad libitum |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001122 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | Ndufs4, https://www.jax.org/strai n/02705 8 |
Age Or Age Range: | 45-50 days |
Gender: | Male |
Animal Animal Supplier: | Jackson Laboratory (ME, USA) |
Animal Light Cycle: | 12:12 h |
Animal Feed: | Rodent Breeder, Cat. #RM1845, LabChef, Nutritionhub |
Animal Water: | ad libitum |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
MQ_29 | SA072619 | FL011381 | Ndufs4-/- | Genotype |
Collection:
Collection ID: | CO001116 |
Collection Summary: | Mice were euthanized between postnatal day (P) 45-50 via cervical dislocation at the same time of day (8:00-9:00 AM) after overnight (12-h) fasting. From both hind limbs, white muscle portions of quadriceps femoris, as well as soleus muscles were collected. Tissues were snap-frozen in liquid nitrogen (within 15 min post-mortem) and stored at -80°C until used. |
Sample Type: | Muscle |
Treatment:
Treatment ID: | TR001136 |
Treatment Summary: | The animals did not receive any treatment |
Sample Preparation:
Sampleprep ID: | SP001129 |
Sampleprep Summary: | Muscle tissues were finely minced and homogenized in the presence of internal standards using a vibration mill. Metabolite extraction was achieved using a modified monophasic Bligh–Dyer extraction method with a solvent ratio of 3:1:1 (methanol:water:chloroform). |
Sampleprep Protocol Filename: | NMR_quadriceps_sample_prep_protocol. pdf |
Analysis:
MB Sample ID: | SA072619 |
Analysis ID: | AN001760 |
Analysis Type: | NMR |
Analysis Protocol File: | NMR_data_acquisition_method.pdf |
Processing Parameters File: | Data_processing_method.pdf |
Num Factors: | 2 |
Num Metabolites: | 36 |
Units: | Normalized peak area |
NMR:
NMR ID: | NM000131 |
Analysis ID: | AN001760 |
Instrument Name: | Bruker Avance III HD |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D 1H |
Spectrometer Frequency: | 500 MHz |