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MB Sample ID: SA073574
Local Sample ID: | 15 |
Subject ID: | SU001133 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | B6Ly5.2 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001133 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | B6Ly5.2 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
15 | SA073574 | FL011424 | Allogenic | BMT Type |
15 | SA073574 | FL011424 | WT | Mice type |
15 | SA073574 | FL011424 | Large Intestine lumen | Site |
Collection:
Collection ID: | CO001127 |
Collection Summary: | Stool lumen contents |
Sample Type: | Intestinal lumen contents |
Treatment:
Treatment ID: | TR001147 |
Treatment Summary: | BMT from naive [B6Ly5.2→B6] and allogenic e allogeneic [B6Ly5.2→Nlrp6-/-] animals |
Sample Preparation:
Sampleprep ID: | SP001140 |
Sampleprep Summary: | To determine targeted taurine quantitation, samples (int estinal fecal content) from mice 21d post-transplant were harvested, homogenized, and snap-frozen in liquid N2. Fecal content was we ighed at necropsy. Samples were extracted with acetonitrile spiked with stable isotope-labeled C13 taurine standards. The supernatan t was analyzed by Agilent 6490 mass spectrometer in a positive electrospray ionization mode after liquid chromatography using a HILI C mode and isocratic gradient. Quantitation was performed by calibration to internal standards and expressed in mM after normalizati on for weights. |
Combined analysis:
Analysis ID | AN001774 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Agilent 1290 Infinity |
Column | Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Agilent 6410 QQQ |
Ion Mode | POSITIVE |
Units | M/100mg stool |
Chromatography:
Chromatography ID: | CH001254 |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um) |
Chromatography Type: | HILIC |
MS:
MS ID: | MS001640 |
Analysis ID: | AN001774 |
Instrument Name: | Agilent 6410 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | POSITIVE |