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MB Sample ID: SA074557
| Local Sample ID: | 12C-AC-Leaf |
| Subject ID: | SU001139 |
| Subject Type: | Plant |
| Subject Species: | Allium cepa;Arabidopsis thaliana;Glycine max;Glycyrrhiza glabra |
| Subject Comments: | Allium cepa/Arabidopsis thaliana/Glycine max/Glycyrrhiza glabra/Glycyrrhiza urahensis/Medicago truncatula/Nicotiana tabacum/Ophiorrhiza pumila/Oryza sativa/Solanum lycopersicum/Solanum tuberosum/Zea mays |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001139 |
| Subject Type: | Plant |
| Subject Species: | Allium cepa;Arabidopsis thaliana;Glycine max;Glycyrrhiza glabra |
| Subject Comments: | Allium cepa/Arabidopsis thaliana/Glycine max/Glycyrrhiza glabra/Glycyrrhiza urahensis/Medicago truncatula/Nicotiana tabacum/Ophiorrhiza pumila/Oryza sativa/Solanum lycopersicum/Solanum tuberosum/Zea mays |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 12C-AC-Leaf | SA074557 | FL011453 | Non-labelled | Label |
| 12C-AC-Leaf | SA074557 | FL011453 | Allium cepa | Species |
| 12C-AC-Leaf | SA074557 | FL011453 | leaf | SampleSource |
Collection:
| Collection ID: | CO001133 |
| Collection Summary: | Leaf/stem and root tissues of Arabidopsis thaliana, Nicotiana tabacum, Glycine max, Glycyrrhiza glabra, Glycyrrhiza urahensis, and Oryza sativa, bulb, leaf, and root tissues of Allium cepa, flower, leaf/stem, seed pod, seed, and root tissues of Medicago truncatula, green and ripe fruit-, and leaf/stem tissues of Solanum lycopersicum, leaf/stem, root, and tuber tissues of Solanum tuberosum, and leaf, stem, seed, and root tissues of Zea mays were purchased from IsoLife Co. (Wageningen UR, Netherlands). The hairy root of Ophiorrhiza pumila was grown as follows. O. pumila hairy roots were derived and maintained, except for the carbon source. We supplied 1.5% D-glucose instead of 1.5% sucrose. We obtained 4-week-old O. pumila hairy roots and sub-cultured them in 1.5% D-glucose (U-13C6, 99%) (Cambridge Isotope Laboratories, Inc.) for two generations for complete isotope labeling of the metabolome. Hairy roots from the same batch used for isotope-labeling were sub-cultured for two subcultures in 1.5% D-glucose (Nacalai Tesque, Inc); they served as the control. Hairy roots from the second sub-culture of sub-cultured tissues growing in 1.5% isotope-labelled or un-labelled 1.5% D-glucose were harvested, wiped with paper towels and snap-frozen in liquid-N2. |
| Sample Type: | Plant |
Treatment:
| Treatment ID: | TR001153 |
| Treatment Summary: | NA |
Sample Preparation:
| Sampleprep ID: | SP001146 |
| Sampleprep Summary: | Samples (3 mg) of dried plant tissues were weighted in a 2.0-ml microtube (Sarstedt). Their metabolites were extracted with 150 µl of 80% MeOH containing 2.5 µM lidocaine and 10-camphour sulfonic acid per mg of dry weight using a mixer-mill with zirconia beads [7 min at 18 Hz and 4 degree C (MM300; Retsch)]. After 10-min centrifugation at 17,800 g the supernatant was filtered using an HLB µElution plate (Waters). |
Combined analysis:
| Analysis ID | AN001781 | AN001782 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Waters Acquity | Waters Acquity |
| Column | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Waters Synapt G2 S QTOF | Waters Synapt G2 S QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak intensity | Peak intensity |
Chromatography:
| Chromatography ID: | CH001259 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 40 |
| Flow Gradient: | 99.5% solvent A/0.5% solvent B at 0 min, 99.5%A/0.5%B at 0.1 min, 20%A/80%B at 10 min, 0.5%A/99.5%B at 10.1 min, 0.5%A/99.5%B at 12.0 min, 99.5%A/0.5%B at 12.1 min and 99.5%A/0.5%B at 15.0 min |
| Flow Rate: | 0.3 ml/min at 0 min, 0.3 ml/min at 10 min, 0.4 ml/min at 10.1 min, 0.4 ml/min at 14.4 min, and 0.3 ml/min at 14.5 min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001645 |
| Analysis ID: | AN001781 |
| Instrument Name: | Waters Synapt G2 S QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Capillary Voltage: | +3.00 kV |
| Collision Energy: | 6 V |
| Source Temperature: | 120 |
| Dataformat: | .raw |
| Desolvation Gas Flow: | 800 l/hr |
| Desolvation Temperature: | 450 degree C |
| Scan Range Moverz: | 50 - 1500 |
| MS ID: | MS001646 |
| Analysis ID: | AN001782 |
| Instrument Name: | Waters Synapt G2 S QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Capillary Voltage: | -2.75 kV |
| Collision Energy: | 6 V |
| Source Temperature: | 120 |
| Dataformat: | .raw |
| Desolvation Gas Flow: | 800 l/hr |
| Desolvation Temperature: | 450 degree C |
| Scan Range Moverz: | 50 - 1500 |
