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MB Sample ID: SA075189
| Local Sample ID: | SL_10.d |
| Subject ID: | SU001149 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001149 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| SL_10.d | SA075189 | FL011634 | Seryl-leucine Standard | Sample Type |
Collection:
| Collection ID: | CO001143 |
| Collection Summary: | Urine was collected and frozen at -20 for storage. Prior to analysis, urine was thawed and centrifuged to remove particulates. |
| Sample Type: | Urine |
Treatment:
| Treatment ID: | TR001163 |
| Treatment Summary: | Urine, SLC1G enriched from urine, and seryl-leucine synthetic standard were analyzed by LC-MS and LC-MS/MS |
Sample Preparation:
| Sampleprep ID: | SP001156 |
| Sampleprep Summary: | Urine was thawed on ice and centrifuged to remove particulates prior to analysis. |
Combined analysis:
| Analysis ID | AN001796 | AN001797 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1200 | Agilent 1200 |
| Column | Atlantis T3 reverse-phase C18 (150 x 2.1mm,3.5um) | Atlantis T3 reverse-phase C18 (150 x 2.1mm,3.5um) |
| MS Type | ESI | ESI |
| MS instrument type | TOF | QTOF |
| MS instrument name | Agilent 6230 TOF | Agilent 6520 QTOF |
| Ion Mode | POSITIVE | POSITIVE |
| Units | counts | m/z |
Chromatography:
| Chromatography ID: | CH001268 |
| Instrument Name: | Agilent 1200 |
| Column Name: | Atlantis T3 reverse-phase C18 (150 x 2.1mm,3.5um) |
| Column Temperature: | 30 |
| Flow Gradient: | 100% Solvent A to 90% Solvent B |
| Flow Rate: | 0.25 ml/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% methanol; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001657 |
| Analysis ID: | AN001796 |
| Instrument Name: | Agilent 6230 TOF |
| Instrument Type: | TOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Capillary Voltage: | 2000 V |
| Dry Gas Flow: | 11 L/min |
| Fragment Voltage: | 120 V |
| Nebulizer: | 40 psi |
| Octpole Voltage: | 750 V |
| Skimmer Voltage: | 60 V |
| Analysis Protocol File: | SLC1G_Identification_Methods.docx |
| MS ID: | MS001658 |
| Analysis ID: | AN001797 |
| Instrument Name: | Agilent 6520 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Capillary Voltage: | 2000 V |
| Collision Energy: | 10 V |
| Dry Gas Flow: | 8 L/min |
| Fragment Voltage: | 120 V |
| Nebulizer: | 45 psi |
| Octpole Voltage: | 750 V |
| Scanning Cycle: | 1.2 spectra/sec |
| Scanning Range: | 100-1700 m/z |
| Skimmer Voltage: | 60 V |
| Analysis Protocol File: | SLC1G_Identification_Methods.docx |
