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MB Sample ID: SA075190
Local Sample ID: | SL-r002.d |
Subject ID: | SU001149 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001149 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
SL-r002.d | SA075190 | FL011634 | Seryl-leucine Standard | Sample Type |
Collection:
Collection ID: | CO001143 |
Collection Summary: | Urine was collected and frozen at -20 for storage. Prior to analysis, urine was thawed and centrifuged to remove particulates. |
Sample Type: | Urine |
Treatment:
Treatment ID: | TR001163 |
Treatment Summary: | Urine, SLC1G enriched from urine, and seryl-leucine synthetic standard were analyzed by LC-MS and LC-MS/MS |
Sample Preparation:
Sampleprep ID: | SP001156 |
Sampleprep Summary: | Urine was thawed on ice and centrifuged to remove particulates prior to analysis. |
Combined analysis:
Analysis ID | AN001796 | AN001797 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Agilent 1200 | Agilent 1200 |
Column | Atlantis T3 reverse-phase C18 (150 x 2.1mm,3.5um) | Atlantis T3 reverse-phase C18 (150 x 2.1mm,3.5um) |
MS Type | ESI | ESI |
MS instrument type | TOF | QTOF |
MS instrument name | Agilent 6230 TOF | Agilent 6520 QTOF |
Ion Mode | POSITIVE | POSITIVE |
Units | counts | m/z |
Chromatography:
Chromatography ID: | CH001268 |
Instrument Name: | Agilent 1200 |
Column Name: | Atlantis T3 reverse-phase C18 (150 x 2.1mm,3.5um) |
Column Temperature: | 30 |
Flow Gradient: | 100% Solvent A to 90% Solvent B |
Flow Rate: | 0.25 ml/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% methanol; 0.1% formic acid |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001657 |
Analysis ID: | AN001796 |
Instrument Name: | Agilent 6230 TOF |
Instrument Type: | TOF |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Capillary Voltage: | 2000 V |
Dry Gas Flow: | 11 L/min |
Fragment Voltage: | 120 V |
Nebulizer: | 40 psi |
Octpole Voltage: | 750 V |
Skimmer Voltage: | 60 V |
Analysis Protocol File: | SLC1G_Identification_Methods.docx |
MS ID: | MS001658 |
Analysis ID: | AN001797 |
Instrument Name: | Agilent 6520 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Capillary Voltage: | 2000 V |
Collision Energy: | 10 V |
Dry Gas Flow: | 8 L/min |
Fragment Voltage: | 120 V |
Nebulizer: | 45 psi |
Octpole Voltage: | 750 V |
Scanning Cycle: | 1.2 spectra/sec |
Scanning Range: | 100-1700 m/z |
Skimmer Voltage: | 60 V |
Analysis Protocol File: | SLC1G_Identification_Methods.docx |