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MB Sample ID: SA075877
Local Sample ID: | M-GroupII_33 |
Subject ID: | SU001157 |
Subject Type: | Fish |
Subject Species: | Megalobrama amblycephala |
Taxonomy ID: | 75352 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001157 |
Subject Type: | Fish |
Subject Species: | Megalobrama amblycephala |
Taxonomy ID: | 75352 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
M-GroupII_33 | SA075877 | FL011667 | 2 | age(years) |
M-GroupII_33 | SA075877 | FL011667 | No | mature |
M-GroupII_33 | SA075877 | FL011667 | No | spawned |
M-GroupII_33 | SA075877 | FL011667 | Yes | hormone treatment |
Collection:
Collection ID: | CO001151 |
Collection Summary: | The gonads of fish were collected to identify its sex of 1-year-old fish by traditional histological analysis and the muscle samples were only selected from the females (group Ⅰ). As to 2-year-old M. amblycephala, the muscle samples were firstly collected from individuals, and then the gonads were collected to do the histological analysis. Individuals with ovary development before stage Ⅴ were recognized as non-mature females (group Ⅱ), while individuals with ovary development at stage Ⅴdwere recognized as mature females (group ⅡI). For group IV, the individuals which normally spawned during artificial reproduction, were put back to tank and then muscle samples were collected after 24 hours. For each group, muscle samples from 10 individuals were collected. After the collection, muscle samples were frozen in liquid nitrogen immediately and then transferred them into -80℃ for further study. |
Sample Type: | Muscle and Gonad tissue |
Treatment:
Treatment ID: | TR001171 |
Treatment Summary: | Samples were divided into four groups: 1-year-old immature M. amblycephala females (group Ⅰ), 2-year-old immature M. amblycephala females (group Ⅰ), 2-year-old mature females before the injection of hormones (group Ⅲ) and 2-year-old females after 24 hours of their successfully spawning by injecting oxytocin (group ⅣanThe artificial oxytocin implementation procedure was as follows. Firstly, the fish were intraperitoneally injected with a luteinizing hormone-releasing hormone from the basal part of the pectoral fin (LRH-A2) (1 µg/kg fish body weight). After 10 h, the second injection was given (0.5 ml/kg body weight) (each ml contained LRH-A2 4 µg, human chorionic gonadotropin (HCG) 500 U, and domperidone (DOM) 5 mg of solution). |
Sample Preparation:
Sampleprep ID: | SP001164 |
Sampleprep Summary: | Muscle tissues about 25 mg from each individual were homogenized in 800 µL cold methanol and water (v/v = 1:1) using a Qiagen Tissue-Lyser (Retsch GmBH, Germany). 400 µL supernatant was collected after centrifugation (25000 g, 4 °C, 10 min). The supernatant in the eppendorf tube was removed and the sediment in the tube was dried. Homogenized the sediment again in 800 µL cold dichloromethane and methanol (v/v = 3:1), after centrifuging and then collected 200 µL supernatant for QC (quality control) sample (25000 g, 4 °C, 10 min). The supernatant was evacuated under vacuum, re-collected 100µL supernatant after centrifugation (25000 g, 4 °C, 10 min) for subsequent detection. |
Combined analysis:
Analysis ID | AN001807 | AN001808 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Unspecified | Unspecified |
Chromatography system | Waters | Waters |
Column | ion information | ion information |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Waters Synapt G2 XS QTOF | Waters Synapt G2 XS QTOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | none | none |
Chromatography:
Chromatography ID: | CH001273 |
Instrument Name: | Waters |
Column Name: | ion information |
Chromatography Type: | Unspecified |
MS:
MS ID: | MS001665 |
Analysis ID: | AN001807 |
Instrument Name: | Waters Synapt G2 XS QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | POSITIVE |
MS ID: | MS001666 |
Analysis ID: | AN001808 |
Instrument Name: | Waters Synapt G2 XS QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | NEGATIVE |