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MB Sample ID: SA077624
Local Sample ID: | 30 |
Subject ID: | SU001174 |
Subject Type: | NMR based metabolomics of microbes |
Subject Species: | Nesterenkonia flava |
Taxonomy ID: | 469799 |
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Subject:
Subject ID: | SU001174 |
Subject Type: | NMR based metabolomics of microbes |
Subject Species: | Nesterenkonia flava |
Taxonomy ID: | 469799 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
30 | SA077624 | FL011805 | 1K00606 | Strain |
30 | SA077624 | FL011805 | Marine | Strain source |
30 | SA077624 | FL011805 | A6 | Medium |
Collection:
Collection ID: | CO001168 |
Collection Summary: | The broth cultures (50 mL) were harvested via centrifugation at 7000 g for 10 min under 4 °C. The pellet was quenched using 10 mL of 60% cold methanol (−80 °C) containing 0.85% (wt./vol.) of NaCl for 30 min. The quenched cell pellets were re-suspended in 10 mL of cold PBS and were washed for 3 times. |
Sample Type: | Cultured cells |
Treatment:
Treatment ID: | TR001188 |
Treatment Summary: | For each cell pellet sample, 5 mL of the mixture of methanol/water (1:0.9, v/v) was added and ultrasonicated for 25 min to break cells bathing in ice. The mixture was added with 5 mL of cold chloroform, vortexed and subjected to 10 min of ultrasonic extraction under the bath of ice. The mixture was then centrifuged at 9500 g for 8 min. The upper layer phase containing methanol and water was taken out and evaporated in the fume cupboard to remove methanol. The remaining water solution was freezed under −80 °C, and then freeze-dried to afford dry samples, which were stored under −80 °C before analysis. |
Sample Preparation:
Sampleprep ID: | SP001181 |
Sampleprep Summary: | The intracellular extract was re-suspended in 550 µL of phosphate buffer containing 1.5 M KH2PO4, 0.1% sodium 3-(trimethylsilyl) propionate-2,2,3,3-d4 (TSP), and 10% D2O. Subsequently, all the samples were vortexed and centrifuged at 12000 g for 15 min at 4°C to remove any insoluble components. The collected supernatants (500 μL) were transferred to 5 mm NMR tubes for analysis. |
Analysis:
MB Sample ID: | SA077624 |
Analysis ID: | AN001828 |
Analysis Type: | NMR |
Num Factors: | 12 |
NMR:
NMR ID: | NM000141 |
Analysis ID: | AN001828 |
Instrument Name: | Bruker Avance III |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D 1H |
Spectrometer Frequency: | 850 MHz |