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MB Sample ID: SA077665
Local Sample ID: | C5 |
Subject ID: | SU001175 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001175 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
C5 | SA077665 | FL011812 | CHG5 | Celltype |
C5 | SA077665 | FL011812 | lowgrade | Treatment |
Collection:
Collection ID: | CO001169 |
Collection Summary: | Muscles were rapidly dissected, weighted and frozen in liquid nitrogen and stored at -80 °C until analyses. |
Sample Type: | Muscle |
Treatment:
Treatment ID: | TR001189 |
Treatment Summary: | Right caudate nuclei of mice were separately injected with 2.0 μL of the suspension of 2.0 × 105 of HEB, CHG5 and U87 cells. muscles were rapidly dissected, weighted and frozen. |
Sample Preparation:
Sampleprep ID: | SP001182 |
Sampleprep Summary: | Generally, gastrocnemius muscle samples were homogenized after adding ice-cold methanol, chloroform and water at a volume ratio of 4:4:2.85 to obtain a two-phase extract. |
Analysis:
MB Sample ID: | SA077665 |
Analysis ID: | AN001829 |
Analysis Type: | NMR |
Num Factors: | 3 |
NMR:
NMR ID: | NM000142 |
Analysis ID: | AN001829 |
Instrument Name: | Bruker Avance III |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D 1H |
Spectrometer Frequency: | 850 MHz |