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MB Sample ID: SA077678
| Local Sample ID: | U3 |
| Subject ID: | SU001175 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001175 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| U3 | SA077678 | FL011814 | U87 | Celltype |
| U3 | SA077678 | FL011814 | highgrade | Treatment |
Collection:
| Collection ID: | CO001169 |
| Collection Summary: | Muscles were rapidly dissected, weighted and frozen in liquid nitrogen and stored at -80 °C until analyses. |
| Sample Type: | Muscle |
Treatment:
| Treatment ID: | TR001189 |
| Treatment Summary: | Right caudate nuclei of mice were separately injected with 2.0 μL of the suspension of 2.0 × 105 of HEB, CHG5 and U87 cells. muscles were rapidly dissected, weighted and frozen. |
Sample Preparation:
| Sampleprep ID: | SP001182 |
| Sampleprep Summary: | Generally, gastrocnemius muscle samples were homogenized after adding ice-cold methanol, chloroform and water at a volume ratio of 4:4:2.85 to obtain a two-phase extract. |
Analysis:
| MB Sample ID: | SA077678 |
| Analysis ID: | AN001829 |
| Analysis Type: | NMR |
| Num Factors: | 3 |
NMR:
| NMR ID: | NM000142 |
| Analysis ID: | AN001829 |
| Instrument Name: | Bruker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Spectrometer Frequency: | 850 MHz |
