Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA080282

Local Sample ID:	NR1
Subject ID:	SU001220
Subject Type:	Plant
Subject Species:	Phoenix dactylifera
Taxonomy ID:	42345

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Subject:

Subject ID:	SU001220
Subject Type:	Plant
Subject Species:	Phoenix dactylifera
Taxonomy ID:	42345

Factors:

Local Sample ID	MB Sample ID	Factor Level ID	Level Value	Factor Name
NR1	SA080282	FL012298	NaCl-Root	Treatment

Collection:

Collection ID:	CO001214
Collection Summary:	Leaf and root tissues were collected from date palm seedlings after treatment with NaCl and/or silicon, flash frozen with liquid nitrogen, and freeze dried.
Sample Type:	Plant

Treatment:

Treatment ID:	TR001235
Treatment Summary:	Once the radicle of the seeds had emerged, they were then transferred to potting compost and maintained at 32C/30C with a photoperiod of 16/8 h in the growth chamber. The seedlings were then divided into four sets based on their treatments and were irrigated either with tap water with an electrical conductivity of 50 mS/m, considered as a control treatment (control), 300 mM NaCl without additional silicon for salt stress (NaCl), 5 mM Na2SiO3 (+Si) for silicon treatment or 5 mM of sodium silicate (Na2SiO3) along with 300 mM NaCl (NaCl+Si) for salt stress and silicon treatment. The NaCl and silicon treatments were administered on a weekly basis, as irrigation.

Treatment ID:

TR001235

Treatment Summary:

Once the radicle of the seeds had emerged, they were then transferred to potting compost and maintained at 32C/30C with a photoperiod of 16/8 h in the growth chamber. The seedlings were then divided into four sets based on their treatments and were irrigated either with tap water with an electrical conductivity of 50 mS/m, considered as a control treatment (control), 300 mM NaCl without additional silicon for salt stress (NaCl), 5 mM Na2SiO3 (+Si) for silicon treatment or 5 mM of sodium silicate (Na2SiO3) along with 300 mM NaCl (NaCl+Si) for salt stress and silicon treatment. The NaCl and silicon treatments were administered on a weekly basis, as irrigation.

Sample Preparation:

Sampleprep ID:	SP001228
Sampleprep Summary:	A total of 24 samples were analyzed using LC-MS by HILIC chromatography on a 0.5 x 150 mm Zic-pHILIC column using 10 mM NH4HCO3 in water (A) and 10 mM NH4HCO3 in 95% acetonitrile (B) as solvents and by RPLC chromatography using the same solvents and a 0.5 x 100 mm PLRPS column. The Samples were extracted at a tissue concentration of 100 mg/mL (leaves) or 50 mg/mL (roots) in 80% methanol by vortexing for 10 minutes. The solids were collected by centrifugation, and then the supernatant was filtered through a 0.8 μm spin filter. Samples were injected onto the LC-MS and data was acquired in polarity switching mode with data dependent acquisition of MS/MS spectra.

Sampleprep ID:

SP001228

Sampleprep Summary:

A total of 24 samples were analyzed using LC-MS by HILIC chromatography on a 0.5 x 150 mm Zic-pHILIC column using 10 mM NH4HCO3 in water (A) and 10 mM NH4HCO3 in 95% acetonitrile (B) as solvents and by RPLC chromatography using the same solvents and a 0.5 x 100 mm PLRPS column. The Samples were extracted at a tissue concentration of 100 mg/mL (leaves) or 50 mg/mL (roots) in 80% methanol by vortexing for 10 minutes. The solids were collected by centrifugation, and then the supernatant was filtered through a 0.8 μm spin filter. Samples were injected onto the LC-MS and data was acquired in polarity switching mode with data dependent acquisition of MS/MS spectra.

Combined analysis:

Analysis ID	AN001911	AN001912
Analysis type	MS	MS
Chromatography type	HILIC	Reversed phase
Chromatography system	Thermo Q-Exactive	Thermo Q-Exactive
Column	Zic-pHILIC 0.5 x 150 mm	0.5 x 100 mm PLRPS column
MS Type	ESI	ESI
MS instrument type	Orbitrap	Orbitrap
MS instrument name	Thermo Q Exactive Orbitrap	Thermo Q Exactive Orbitrap
Ion Mode	UNSPECIFIED	UNSPECIFIED
Units	log10(peak area)	log10(peak area)

Chromatography:

Chromatography ID:	CH001384
Instrument Name:	Thermo Q-Exactive
Column Name:	Zic-pHILIC 0.5 x 150 mm
Chromatography Type:	HILIC

Chromatography ID:	CH001385
Instrument Name:	Thermo Q-Exactive
Column Name:	0.5 x 100 mm PLRPS column
Chromatography Type:	Reversed phase

MS:

MS ID:	MS001767
Analysis ID:	AN001911
Instrument Name:	Thermo Q Exactive Orbitrap
Instrument Type:	Orbitrap
MS Type:	ESI
MS Comments:	Samples were injected onto the LC-MS and data was acquired in polarity switching mode with data dependent acquisition of MS/MS spectra. The ion mode is polarity-switching, which means it scans in positive and negative ion mode during the sample run, switching between the two modes about every 20 milliseconds.
Ion Mode:	UNSPECIFIED

MS ID:	MS001768
Analysis ID:	AN001912
Instrument Name:	Thermo Q Exactive Orbitrap
Instrument Type:	Orbitrap
MS Type:	ESI
MS Comments:	Samples were injected onto the LC-MS and data was acquired in polarity switching mode with data dependent acquisition of MS/MS spectra. The ion mode is polarity-switching, which means it scans in positive and negative ion mode during the sample run, switching between the two modes about every 20 milliseconds.
Ion Mode:	UNSPECIFIED