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MB Sample ID: SA080304
Local Sample ID: | MTLZ-1 |
Subject ID: | SU001221 |
Subject Type: | Plant |
Subject Species: | Phoenix dactylifera |
Taxonomy ID: | 42345 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001221 |
Subject Type: | Plant |
Subject Species: | Phoenix dactylifera |
Taxonomy ID: | 42345 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
MTLZ-1 | SA080304 | FL012305 | Treated leaf Zabad | Factor |
Collection:
Collection ID: | CO001215 |
Collection Summary: | Leaf and root tissues of Umsila and Zabad cultivars were collected after irrigation either with water or NaCl solution. |
Sample Type: | Plant |
Treatment:
Treatment ID: | TR001236 |
Treatment Summary: | Seedlings were either irrigated with water or NaCl solution for about 2 months. |
Sample Preparation:
Sampleprep ID: | SP001229 |
Sampleprep Summary: | Metabolomics and basic data analysis were carried out for service charges at proteomics and mass spectrometry facility, Donald Danforth Plant Science Centre, Sant Louis, USA, using liquid chromatography mass spectrometry. Briefly, a total of 24 samples were analyzed for polar metabolites using hydrophilic interaction liquid chromatography (HILIC) and nonpolar metabolites using reverse phase liquid chromatography (RPLC). The Samples were extracted in 80% methanol at a tissue concentration of 100 mg/mL by a vortex for 10 minutes. The solids were collected by centrifugation, and then the supernatant was filtered through a 0.8 μm spin filter. The extracts were injected into the LC-MS and the data was acquired in polarity switching mode with data dependent acquisition of MS/MS spectra. |
Combined analysis:
Analysis ID | AN001913 | AN001914 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | Reversed phase |
Chromatography system | Thermo Q-Exactive | Thermo Q-Exactive |
Column | chromatography on a 0.5 x 150mm | 0.5 x 100 mm PLRPS column |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | UNSPECIFIED | UNSPECIFIED |
Units | log10(peak area) | log10(peak area) |
Chromatography:
Chromatography ID: | CH001386 |
Instrument Name: | Thermo Q-Exactive |
Column Name: | chromatography on a 0.5 x 150mm |
Chromatography Type: | HILIC |
Chromatography ID: | CH001387 |
Instrument Name: | Thermo Q-Exactive |
Column Name: | 0.5 x 100 mm PLRPS column |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001769 |
Analysis ID: | AN001913 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | The ion mode is polarity-switching, which means it scans in positive and negative ion mode during the sample run, switching between the two modes about every 20 milliseconds. |
Ion Mode: | UNSPECIFIED |
MS ID: | MS001770 |
Analysis ID: | AN001914 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | The ion mode is polarity-switching, which means it scans in positive and negative ion mode during the sample run, switching between the two modes about every 20 milliseconds. |
Ion Mode: | UNSPECIFIED |