Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA080309

Local Sample ID:	MTRZ-3
Subject ID:	SU001221
Subject Type:	Plant
Subject Species:	Phoenix dactylifera
Taxonomy ID:	42345

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Subject:

Subject ID:	SU001221
Subject Type:	Plant
Subject Species:	Phoenix dactylifera
Taxonomy ID:	42345

Factors:

Local Sample ID	MB Sample ID	Factor Level ID	Level Value	Factor Name
MTRZ-3	SA080309	FL012307	Treated root Zabad	Factor

Collection:

Collection ID:	CO001215
Collection Summary:	Leaf and root tissues of Umsila and Zabad cultivars were collected after irrigation either with water or NaCl solution.
Sample Type:	Plant

Treatment:

Treatment ID:	TR001236
Treatment Summary:	Seedlings were either irrigated with water or NaCl solution for about 2 months.

Sample Preparation:

Sampleprep ID:	SP001229
Sampleprep Summary:	Metabolomics and basic data analysis were carried out for service charges at proteomics and mass spectrometry facility, Donald Danforth Plant Science Centre, Sant Louis, USA, using liquid chromatography mass spectrometry. Briefly, a total of 24 samples were analyzed for polar metabolites using hydrophilic interaction liquid chromatography (HILIC) and nonpolar metabolites using reverse phase liquid chromatography (RPLC). The Samples were extracted in 80% methanol at a tissue concentration of 100 mg/mL by a vortex for 10 minutes. The solids were collected by centrifugation, and then the supernatant was filtered through a 0.8 μm spin filter. The extracts were injected into the LC-MS and the data was acquired in polarity switching mode with data dependent acquisition of MS/MS spectra.

Sampleprep ID:

SP001229

Sampleprep Summary:

Metabolomics and basic data analysis were carried out for service charges at proteomics and mass spectrometry facility, Donald Danforth Plant Science Centre, Sant Louis, USA, using liquid chromatography mass spectrometry. Briefly, a total of 24 samples were analyzed for polar metabolites using hydrophilic interaction liquid chromatography (HILIC) and nonpolar metabolites using reverse phase liquid chromatography (RPLC). The Samples were extracted in 80% methanol at a tissue concentration of 100 mg/mL by a vortex for 10 minutes. The solids were collected by centrifugation, and then the supernatant was filtered through a 0.8 μm spin filter. The extracts were injected into the LC-MS and the data was acquired in polarity switching mode with data dependent acquisition of MS/MS spectra.

Combined analysis:

Analysis ID	AN001913	AN001914
Analysis type	MS	MS
Chromatography type	HILIC	Reversed phase
Chromatography system	Thermo Q-Exactive	Thermo Q-Exactive
Column	chromatography on a 0.5 x 150mm	0.5 x 100 mm PLRPS column
MS Type	ESI	ESI
MS instrument type	Orbitrap	Orbitrap
MS instrument name	Thermo Q Exactive Orbitrap	Thermo Q Exactive Orbitrap
Ion Mode	UNSPECIFIED	UNSPECIFIED
Units	log10(peak area)	log10(peak area)

Chromatography:

Chromatography ID:	CH001386
Instrument Name:	Thermo Q-Exactive
Column Name:	chromatography on a 0.5 x 150mm
Chromatography Type:	HILIC

Chromatography ID:	CH001387
Instrument Name:	Thermo Q-Exactive
Column Name:	0.5 x 100 mm PLRPS column
Chromatography Type:	Reversed phase

MS:

MS ID:	MS001769
Analysis ID:	AN001913
Instrument Name:	Thermo Q Exactive Orbitrap
Instrument Type:	Orbitrap
MS Type:	ESI
MS Comments:	The ion mode is polarity-switching, which means it scans in positive and negative ion mode during the sample run, switching between the two modes about every 20 milliseconds.
Ion Mode:	UNSPECIFIED

MS ID:	MS001770
Analysis ID:	AN001914
Instrument Name:	Thermo Q Exactive Orbitrap
Instrument Type:	Orbitrap
MS Type:	ESI
MS Comments:	The ion mode is polarity-switching, which means it scans in positive and negative ion mode during the sample run, switching between the two modes about every 20 milliseconds.
Ion Mode:	UNSPECIFIED