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MB Sample ID: SA080481
| Local Sample ID: | 0-7_1_1_vq |
| Subject ID: | SU001227 |
| Subject Type: | Other |
| Subject Species: | Homo sapiens |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001227 |
| Subject Type: | Other |
| Subject Species: | Homo sapiens |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 0-7_1_1_vq | SA080481 | FL012326 | food and human plasma | Sample.Composition |
Collection:
| Collection ID: | CO001221 |
| Collection Summary: | Commercially available |
| Sample Type: | Blood (plasma) |
Treatment:
| Treatment ID: | TR001242 |
| Treatment Summary: | Samples were prepared by mixing commercially available vegetable juice and human plsama Qstandard. Smaples were mixed in the ratio of vegetable jucie to plasma at 1024:1, 256:1,64:1, 16:1, 4:1, 1:1, 1:4, 1:16, 1:256, 1:1024. Prior to analysis, samples were stored in -80 degree C. |
Sample Preparation:
| Sampleprep ID: | SP001235 |
| Sampleprep Summary: | Samples were prepared for metabolomics analysis using established methods(Johnson et al. (2010). Analyst; Go et al. (2015). Tox Sci). Prior to analysis, plasma aliquots were removed from storage at -80 degrees C and thawed on ice. Each cryotube was then vortexed briefly to ensure homogeneity, and 50 microliters was transferred to a clean microfuge tube. Immediately after, the plasma was treated with 100 microliters of ice-cold LC-MS grade acetonitrile(Sigma Aldrich) containing 2.5 microliters of internal standard solution with eleven stable isotopic chemicals selected to cover a range of chemical properties. Following addition of acetonitrile, samples were equilibrated for 30 min on ice, upon which precipitated proteins were removed by centrifuge (14,000rpm at 4 degrees C for 10 min). The resulting supernatant (100 microliters) was removed, added to a low volume autosampler vial and maintained at 4 degrees C until analysis (<22 h). |
Combined analysis:
| Analysis ID | AN001921 | AN001922 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | Reversed phase |
| Chromatography system | Agilent 6560 | Agilent 6560 |
| Column | Waters XBridge Amide (100 x 4.6mm,3.5um) | Thermo Accucore C18 (100 x 2.1mm,2.6um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6560 Ion Mobility | Agilent 6560 Ion Mobility |
| Ion Mode | POSITIVE | POSITIVE |
| Units | Peak Area | Peak Area |
Chromatography:
| Chromatography ID: | CH001394 |
| Instrument Name: | Agilent 6560 |
| Column Name: | Waters XBridge Amide (100 x 4.6mm,3.5um) |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH001395 |
| Instrument Name: | Agilent 6560 |
| Column Name: | Thermo Accucore C18 (100 x 2.1mm,2.6um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001777 |
| Analysis ID: | AN001921 |
| Instrument Name: | Agilent 6560 Ion Mobility |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Convert to mzXML format using MSConvert; XCMS R package was used to extract feature table |
| Ion Mode: | POSITIVE |
| MS ID: | MS001778 |
| Analysis ID: | AN001922 |
| Instrument Name: | Agilent 6560 Ion Mobility |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Convert to mzXML format using MSConvert; XCMS R package was used to extract feature table |
| Ion Mode: | POSITIVE |
