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MB Sample ID: SA080735
| Local Sample ID: | af9 |
| Subject ID: | SU001233 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001233 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| af9 | SA080735 | FL012353 | A | Group |
Collection:
| Collection ID: | CO001227 |
| Collection Summary: | Fecal samples were collected from each participant, immediately frozen at −20 °C, transported on ice to the laboratory and then stored at −80 °C. |
| Sample Type: | Feces |
Treatment:
| Treatment ID: | TR001248 |
| Treatment Summary: | To explore how the host metabolic pattern alterations were impacted by the gut microbiota dysbiosis in AF patients, serum samples were collected and analyzed by high-throughput liquid chromatography-mass spectrometry (LC/MS). |
Sample Preparation:
| Sampleprep ID: | SP001241 |
| Sampleprep Summary: | 50 mg fecal samples were pipetted into centrifuge tubes (1.5 mL) in preparation for extraction. The protein was precipitated with 800 μL of methanol and 10 μL of internal standard (2.9 mg/mL, DL-o-Chlorophenylalanine) was added. The samples were ground at 65 KHz for 90 s and centrifuged at 12000 rpm for 15 min at 4 °C. 200 μL of the supernatant was transferred into a vial for further analysis. |
Combined analysis:
| Analysis ID | AN001931 | AN001932 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Thermo Hypergod C18 (100 x 4.6mm,3um) | Thermo Hypergod C18 (100 x 4.6mm,3um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap | Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | feature area | feature area |
Chromatography:
| Chromatography ID: | CH001402 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Thermo Hypergod C18 (100 x 4.6mm,3um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001787 |
| Analysis ID: | AN001931 |
| Instrument Name: | Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | All metabolomic data were prepared for feature extraction and preprocessed with Compound Discoverer 2.0 software (Thermo). Using SIMCA-P software (Umetrics AB, Umea, Sweden), a multivariate Analysis (MVA) was performed. The exact molecular mass, ppm and ms/ms value of these compounds was used to identify the metabolites related to the featured peak in the Metlin database (http://metlin.scripps.edu). The score value indicated the matching rate was calculated by Compound Discoverer 2.0 software (Thermo) with max of 100. |
| Ion Mode: | POSITIVE |
| MS ID: | MS001788 |
| Analysis ID: | AN001932 |
| Instrument Name: | Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | All metabolomic data were prepared for feature extraction and preprocessed with Compound Discoverer 2.0 software (Thermo). Using SIMCA-P software (Umetrics AB, Umea, Sweden), a multivariate Analysis (MVA) was performed. The exact molecular mass, ppm and ms/ms value of these compounds was used to identify the metabolites related to the featured peak in the Metlin database (http://metlin.scripps.edu). The score value indicated the matching rate was calculated by Compound Discoverer 2.0 software (Thermo) with max of 100. |
| Ion Mode: | NEGATIVE |
