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MB Sample ID: SA080842
Local Sample ID: | A49 |
Subject ID: | SU001234 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001234 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
A49 | SA080842 | FL012356 | C | Group |
Collection:
Collection ID: | CO001228 |
Collection Summary: | Blood samples were collected from each participant, immediately frozen at −20 °C, transported on ice to the laboratory and then stored at −80 °C. |
Sample Type: | Blood (serum) |
Treatment:
Treatment ID: | TR001249 |
Treatment Summary: | To explore how the host metabolic pattern alterations were impacted by the gut microbiota dysbiosis in AF patients, serum samples were collected and analyzed by high-throughput liquid chromatography-mass spectrometry (LC/MS). |
Sample Preparation:
Sampleprep ID: | SP001242 |
Sampleprep Summary: | The serum samples were thawed at room temperature and 100 μL was pipetted into centrifuge tubes (1.5 mL) in preparation for extraction. The protein was precipitated with 300 μL of methanol, and 10 μL of internal standard (2.9 mg/mL, DL-o-Chlorophenylalanine) was added. The samples were vortexed for 30 s and centrifuged at 12000 rpm for 15 minat 4 °C. 200 μL of the supernatant was transferred to a vial for further analysis. |
Combined analysis:
Analysis ID | AN001933 | AN001934 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
Column | Thermo Hypergod C18 (100 x 4.6mm,3um) | Thermo Hypergod C18 (100 x 4.6mm,3um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap | Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | feature area | feature area |
Chromatography:
Chromatography ID: | CH001403 |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Thermo Hypergod C18 (100 x 4.6mm,3um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001789 |
Analysis ID: | AN001933 |
Instrument Name: | Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | All metabolomic data were prepared for feature extraction and preprocessed with Compound Discoverer 2.0 software (Thermo). Using SIMCA-P software (Umetrics AB, Umea, Sweden), a multivariate Analysis (MVA) was performed. The exact molecular mass, ppm and ms/ms value of these compounds was used to identify the metabolites related to the featured peak in the Metlin database (http://metlin.scripps.edu). The score value indicated the matching rate was calculated by Compound Discoverer 2.0 software (Thermo) with max of 100. |
Ion Mode: | POSITIVE |
MS ID: | MS001790 |
Analysis ID: | AN001934 |
Instrument Name: | Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | All metabolomic data were prepared for feature extraction and preprocessed with Compound Discoverer 2.0 software (Thermo). Using SIMCA-P software (Umetrics AB, Umea, Sweden), a multivariate Analysis (MVA) was performed. The exact molecular mass, ppm and ms/ms value of these compounds was used to identify the metabolites related to the featured peak in the Metlin database (http://metlin.scripps.edu). The score value indicated the matching rate was calculated by Compound Discoverer 2.0 software (Thermo) with max of 100. |
Ion Mode: | NEGATIVE |