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MB Sample ID: SA081599
Local Sample ID: | 20180922_sample_01 |
Subject ID: | SU001243 |
Subject Type: | Other |
Subject Species: | Cricetulus griseus |
Taxonomy ID: | 10029 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001243 |
Subject Type: | Other |
Subject Species: | Cricetulus griseus |
Taxonomy ID: | 10029 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
20180922_sample_01 | SA081599 | FL012445 | Golgi membrane sample | Sample Type |
Collection:
Collection ID: | CO001237 |
Collection Summary: | Golgi membrane were prepared from Chinese hamster ovary cell line as described previously. J Cell Biol 159, 69-78 (2002); EMBO J 24, 4133-4143 (2005). |
Sample Type: | CHO cells |
Treatment:
Treatment ID: | TR001258 |
Treatment Summary: | Golgi membrane were prepared as described previously. J Cell Biol 159, 69-78 (2002); EMBO J 24, 4133-4143 (2005). |
Sample Preparation:
Sampleprep ID: | SP001251 |
Sampleprep Summary: | 5 mg of Golgi membrane fraction was extracted using acidified CHCl3/methanol (1:2) |
Combined analysis:
Analysis ID | AN001953 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 |
Column | ACQUITY BEH C8 column (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | Peak area |
Chromatography:
Chromatography ID: | CH001416 |
Chromatography Summary: | Lipid extracts were separated on a Waters ACQUITY BEH C8 column (2.1 × 100 mm, 1.7 μm) with the temperature maintained at 40 °C. The flow rate was 250 μL/min, and the mobile phases were consisted of 60:40 water/acetonitrile (A), and 90:10 isopropanol/acetonitrile (B), both containing 10 mM ammonium formate and 0.1% formic acid. The samples were eluted with a linear gradient from 5 % B to 97 % B over 20 min, maintained at 97 % B for 4 min and re-equilibration with 5 % B for 6 min. The sample injection volume was 5 μL |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | ACQUITY BEH C8 column (100 x 2.1mm,1.7um) |
Column Temperature: | 40 |
Flow Gradient: | a linear gradient from 5 % B to 97 % B over 20 min, maintained at 97 % B for 4 min and re-equilibration with 5 % B for 6 min. |
Flow Rate: | 250 µL/min |
Solvent A: | 60% water/40% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001808 |
Analysis ID: | AN001953 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Data analysis and accurate mass calculation were performed using the software Xcalibur 4.0 |
Ion Mode: | POSITIVE |