Return to study ST001178 main page
MB Sample ID: SA081600
Local Sample ID: | Control 1 |
Subject ID: | SU001244 |
Subject Type: | Cultured cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001244 |
Subject Type: | Cultured cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Control 1 | SA081600 | FL012446 | control | treatment |
Collection:
Collection ID: | CO001238 |
Collection Summary: | Cells were washed with cold PBS and then flash-frozen in liquid N2. |
Sample Type: | Muscle |
Treatment:
Treatment ID: | TR001259 |
Treatment Summary: | Two days after confluence, cells were treated with tamoxifen for 24 h and used for the metabolomic analysis. |
Sample Preparation:
Sampleprep ID: | SP001252 |
Sampleprep Summary: | The cells treated with vehicle (control, N = 3) or tamoxifen (FOXO1-ER, N = 3) were used for the metabolomic analysis (Human Metabolome Technologies Inc., Tsuruoka, Japan). 3.9 x 106 cells were transferred into 500 μL of methanol containing 50 mM external standard. After homogenization by BMSM10N21 (BMS, Tokyo) at 1,500 rpm for 120 s performed 5 times, 500 μL of chloroform and 200 μL of ultrapure water were added to the homogenate. The solution was mixed well and centrifuged at 2,300 × g for 5 min at 4 °C. The resultant water phase was ultrafiltered using a Millipore Ultrafree-MC PLHCC HMT Centrifugal Filter Device, 5 kDa (Millipore, Billerica, MA). The filtrate was dried and then dissolved in 50 μL of ultrapure water. The samples obtained were then subjected to capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) analysis using the Agilent CE-TOFMS system (Agilent Technologies, Santa Clara, CA) at 4 °C. The detected peaks were aligned according to their m/z values and normalized migration times. The peaks were mean-centered and scaled using their standard deviations on a per peak basis as a pretreatment. |
Combined analysis:
Analysis ID | AN001954 | AN001955 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | None (Direct infusion) | None (Direct infusion) |
Chromatography system | none | none |
Column | none | none |
MS Type | ESI | ESI |
MS instrument type | TOF | TOF |
MS instrument name | Agilent CE-TOFMS | Agilent CE-TOFMS |
Ion Mode | POSITIVE | NEGATIVE |
Units | Relative Area | fold |
Chromatography:
Chromatography ID: | CH001417 |
Instrument Name: | none |
Column Name: | none |
Chromatography Type: | None (Direct infusion) |
MS:
MS ID: | MS001809 |
Analysis ID: | AN001954 |
Instrument Name: | Agilent CE-TOFMS |
Instrument Type: | TOF |
MS Type: | ESI |
MS Comments: | - |
Ion Mode: | POSITIVE |
MS ID: | MS001810 |
Analysis ID: | AN001955 |
Instrument Name: | Agilent CE-TOFMS |
Instrument Type: | TOF |
MS Type: | ESI |
MS Comments: | - |
Ion Mode: | NEGATIVE |