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MB Sample ID: SA082504
Local Sample ID: | 58h_Control_n=2_2 |
Subject ID: | SU001255 |
Subject Type: | Cultured cells |
Subject Species: | Plasmodium falciparum |
Taxonomy ID: | 5833 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001255 |
Subject Type: | Cultured cells |
Subject Species: | Plasmodium falciparum |
Taxonomy ID: | 5833 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
58h_Control_n=2_2 | SA082504 | FL012547 | Control | Treatment |
58h_Control_n=2_2 | SA082504 | FL012547 | 58 | Time (hr) |
Collection:
Collection ID: | CO001249 |
Collection Summary: | 1x108 Cells per technical replicate were aliquoted into an individual microcentrifuge tube, centrifuged, washed once with 1mL ice-cold PBS, centrifuged, and metabolites extracted with 150uL 80% acetonitrile |
Sample Type: | Cultured cells |
Treatment:
Treatment ID: | TR001270 |
Treatment Summary: | Prior to metabolite extraction, P. falciparum-infected human erythrocyte cultures were treated/untreated with indolmycin |
Sample Preparation:
Sampleprep ID: | SP001263 |
Sampleprep Summary: | Following metabolite extraction with 80% acetontrile. Samples were centrifuged at top speed for 5 minutes and the supernatant collected. Metabolite samples were then stored at -80 until ready for LC-MS analysis |
Combined analysis:
Analysis ID | AN001980 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Agilent 6550 |
Column | SeQuant ZIC-HILIC (150 x 4.6mm,3.5um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Agilent 6550 QTOF |
Ion Mode | NEGATIVE |
Units | ion count |
Chromatography:
Chromatography ID: | CH001429 |
Methods Filename: | pHILIC 45 minute |
Instrument Name: | Agilent 6550 |
Column Name: | SeQuant ZIC-HILIC (150 x 4.6mm,3.5um) |
Column Pressure: | 50-150 bar |
Column Temperature: | 35 |
Flow Gradient: | 80% |
Flow Rate: | 0.3 mL/minute |
Injection Temperature: | 4 |
Solvent A: | 100% water; 20 mM ammonium formate |
Solvent B: | 100% acetonitrile |
Chromatography Type: | HILIC |
MS:
MS ID: | MS001834 |
Analysis ID: | AN001980 |
Instrument Name: | Agilent 6550 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Full m/z scan range across 2-35 minutes. Extended dynamic range, 0-1700 m/z |
Ion Mode: | NEGATIVE |