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MB Sample ID: SA084804
Local Sample ID: | T6 |
Subject ID: | SU001274 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL6/J |
Age Or Age Range: | 12 weeks old |
Weight Or Weight Range: | 25-30 g |
Gender: | Male |
Animal Animal Supplier: | Jax Laboratories |
Animal Housing: | Conventional |
Animal Light Cycle: | regular 12h light /dark cycles |
Animal Feed: | ad libitum normal chow diet |
Animal Water: | ad libitum |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001274 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL6/J |
Age Or Age Range: | 12 weeks old |
Weight Or Weight Range: | 25-30 g |
Gender: | Male |
Animal Animal Supplier: | Jax Laboratories |
Animal Housing: | Conventional |
Animal Light Cycle: | regular 12h light /dark cycles |
Animal Feed: | ad libitum normal chow diet |
Animal Water: | ad libitum |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
T6 | SA084804 | FL012822 | 22C | Temperature |
T6 | SA084804 | FL012822 | DMSO | Treatment |
Collection:
Collection ID: | CO001268 |
Collection Summary: | Mice were anaesthetised with Avertine and blood was collected through cardiac puncture. Samples were let in room temperature for 30 minutes, and then centrifuged for 3 minutes at 14,000RPM. Serum fas collected and frozen at -20C for further lipidomics analysis. |
Sample Type: | Blood (serum) |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR001289 |
Treatment Summary: | Mice were injected i.p. 15 minutes before the cold exposure, with 50ul of DMSO (vehicle) or LOXBlock-1. Then, the animas were placed in 5C or 22C temperature for 4 hours, until the samples were collected. |
Sample Preparation:
Sampleprep ID: | SP001282 |
Sampleprep Summary: | Aliquots of 100 µL serum were taken and a mixture of deuterium-labeled internal standards was added to each aliquot, followed by 3x volume of sample of cold methanol (MeOH). Samples were vortexed for 5 minutes and stored at −20 °C overnight. Cold samples were centrifuged at 14,000g for 10 minutes, and the supernatant was then transferred to a new tube and 3 mL of acidified H2O (pH 3.5) was added to each sample prior to C18 SPE. The methyl formate fractions were collected, dried under nitrogen, and reconstituted in 50 µL MeOH:H2O (1:1, by vol). Samples were transferred to 0.5 mL tubes and centrifuged at 20,000g at 4 °C for 10 minutes. (35ul) of supernatant was transferred to LC–MS/MS vials for analysis using the BERG LC–MS/MS mediator lipidomics platform. |
Combined analysis:
Analysis ID | AN002009 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Ekspert MicroLC 200 system |
Column | Phenomenex Synergi Fusion-RP capillary C18 (150 × 0.5 mm,4um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | ABI Sciex 5600+ TripleTOF |
Ion Mode | NEGATIVE |
Units | Peak Area |
Chromatography:
Chromatography ID: | CH001453 |
Instrument Name: | Ekspert MicroLC 200 system |
Column Name: | Phenomenex Synergi Fusion-RP capillary C18 (150 × 0.5 mm,4um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001862 |
Analysis ID: | AN002009 |
Instrument Name: | ABI Sciex 5600+ TripleTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | MS spectra were acquired in high-resolution mode (>30,000) using a 100-ms accumulation time per spectrum. Full-scan MS/MS was acquired in high sensitivity mode, with an accumulation time optimized per cycle. Collision energy was set using rolling collision energy with a spread of 15V. The identity of a component was confirmed using PeakView® software (SCIEX), and quantification was performed using MultiQuant™ software (SCIEX). C18SPE cartridges were purchased from Biotage. All solvents were of HPLC or LC-MS/MS grade and were acquired from Sigma-Aldrich, Fisher Scientific, or VWR International. |
Ion Mode: | NEGATIVE |