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MB Sample ID: SA084849
| Local Sample ID: | AF10 |
| Subject ID: | SU001275 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001275 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| AF10 | SA084849 | FL012825 | AF | Factors |
Collection:
| Collection ID: | CO001269 |
| Collection Summary: | Fresh stool samples were collected from each participant, immediately frozen at −20 °C, transported on ice to the laboratory and then stored at −80 °C. |
| Sample Type: | Feces |
Treatment:
| Treatment ID: | TR001290 |
| Treatment Summary: | We enrolled participants consisting of patients with AF. |
Sample Preparation:
| Sampleprep ID: | SP001283 |
| Sampleprep Summary: | 20 mg fecal samples were pipetted into centrifuge tubes (1.5 mL) in preparation for extraction. The protein was precipitated with 800 μL of methanol and 10 μL of internal standard (2.9 mg/mL, DL-o-Chlorophenylalanine) was added. The samples were ground at 65 KHz for 90 s and centrifuged at 12000 rpm for 15 min at 4 °C. 200 μL of the supernatant was transferred into a vial for further analysis. |
Combined analysis:
| Analysis ID | AN002010 | AN002011 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Hypersil Gold C18 | Hypersil Gold C18 |
| MS Type | ESI | ESI |
| MS instrument type | Ion trap | Ion trap |
| MS instrument name | Thermo Deca LCQ | Thermo Deca LCQ |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | pg | pg |
Chromatography:
| Chromatography ID: | CH001454 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Hypersil Gold C18 |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001863 |
| Analysis ID: | AN002010 |
| Instrument Name: | Thermo Deca LCQ |
| Instrument Type: | Ion trap |
| MS Type: | ESI |
| MS Comments: | Compound Discoverer 2.0 software (Thermo) |
| Ion Mode: | POSITIVE |
| MS ID: | MS001864 |
| Analysis ID: | AN002011 |
| Instrument Name: | Thermo Deca LCQ |
| Instrument Type: | Ion trap |
| MS Type: | ESI |
| MS Comments: | Compound Discoverer 2.0 software (Thermo) |
| Ion Mode: | NEGATIVE |
